FastQCFastQC Report
Mon 9 Nov 2020
HTHGWDRXX_n02_DJ64-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHGWDRXX_n02_DJ64-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95173200
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCACG270250.026.0044161
GATCGCG113150.025.3225461
GATCAAC703400.024.3729921
GATCGTG312450.023.6936281
GATCCGC176350.023.6572191
GATCCCG199450.022.9422951
GATCCGG210700.022.4615351
GATCCAC418650.022.189131
GATCCTG337800.021.845221
GATCATG508100.020.9760991
GATCCAG406300.020.6064761
GATCGCA254000.020.241291
GATCGTC404350.019.6952361
GATCCTT667150.019.6860771
GATCCGA376550.019.2690811
GATCCCA336900.019.2661231
GATCCGT282800.019.020051
GATCGCC187000.018.345951
GATCACA676400.018.2788261
GATCGCT214400.017.9959951
Produced by FastQC (version 0.11.5)