FastQCFastQC Report
Mon 9 Nov 2020
HTHGWDRXX_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHGWDRXX_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37781228
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGG43400.026.865211
GATCACG55200.025.3295651
GATCCCG43900.024.2919731
GATCAAC140800.023.9338051
GATCCGC39150.023.4863111
GATCGTG61400.023.4665931
GATCGCG29200.020.7764781
GATCCTG67750.020.707481
GATCCAG82900.020.3535561
GATCAGC71850.020.3174291
GATCCCT49050.020.2920111
GATCCGA75550.020.1379571
GATCCTC87800.020.1353471
GATCTGC61450.020.0537821
GATCATG97700.019.9384821
GATCGTC76300.019.815771
GATCCAC84750.019.7414861
GATCGCT49400.019.6685241
GATCCTT136450.019.000221
GATCGCA56350.018.9248921
Produced by FastQC (version 0.11.5)