FastQCFastQC Report
Mon 9 Nov 2020
HTHGWDRXX_n01_DJ74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHGWDRXX_n01_DJ74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257157731
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCACG742450.024.8046131
GATCGCG328750.024.598561
GATCCGC501200.023.5670871
GATCGTG867400.023.044591
GATCAAC1847900.022.1432531
GATCCCG581000.022.104761
GATCCGG582350.021.2621041
GATCGTC1060050.021.0214351
GATCCTG922900.020.1297321
GATCCAC1149450.019.9362321
GATCCAG1096550.019.741091
GATCATG1354550.019.593941
GATCAGC994650.019.0024831
GATCGCC540250.018.7942771
GATCGCT605050.018.7132261
GATCCTC1168150.018.6856521
GATCCGA1044150.018.583981
GATCTGC850200.018.5593871
GATCTCG928400.018.4188561
GATCCGT775150.018.4132351
Produced by FastQC (version 0.11.5)