FastQCFastQC Report
Mon 9 Nov 2020
HTHGWDRXX_n01_DJ73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHGWDRXX_n01_DJ73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342186330
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC660650.026.8476181
GATCACG970450.026.282011
GATCGCG443050.026.0491751
GATCGTG1160150.025.1749321
GATCCGG778000.024.37981
GATCCCG761000.024.01911
GATCAAC2440900.023.7344841
GATCCTG1198800.022.524241
GATCCAG1411850.021.86471
GATCGTC1403200.021.3628981
GATCCAC1504700.020.9481071
GATCAGC1294900.020.8416141
GATCCGA1383550.020.7322061
GATCTGC1115750.020.6875861
GATCATG1798400.020.6578031
GATCCTC1553850.020.4995421
GATCCGT1019400.020.3543741
GATCGCT812200.020.0772171
GATCTCG1212750.019.8595871
GATCGCC705850.019.7297951
Produced by FastQC (version 0.11.5)