FastQCFastQC Report
Mon 9 Nov 2020
HTHGWDRXX_n01_DJ72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHGWDRXX_n01_DJ72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131380933
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC260400.025.234891
GATCACG379750.025.011241
GATCGCG172800.023.2894861
GATCAAC962750.022.5440941
GATCGTG439100.022.3881021
GATCCGG302350.022.2050841
GATCCCG294450.021.364691
GATCCAG552200.020.3667661
GATCCTG470650.020.352261
GATCGTC539700.019.9140871
GATCCAC599300.019.8919141
GATCCGA537000.019.3063741
GATCCGT395350.019.1930221
GATCATG688650.019.0979941
GATCGCA365700.018.6053681
GATCTGC429500.018.5187851
GATCCTC601300.018.4271241
GATCAGC511550.018.2699131
GATCCTT926900.017.9874951
GATCACC442500.017.9103051
Produced by FastQC (version 0.11.5)