FastQCFastQC Report
Mon 9 Nov 2020
HTHGWDRXX_n01_DJ71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHGWDRXX_n01_DJ71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142627944
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCACG388100.027.5460321
GATCCGC256550.027.150771
GATCGCG175850.026.6142371
GATCGTG448700.025.3506011
GATCAAC963400.025.0490781
GATCCCG302750.024.4827751
GATCCGG298000.023.9641971
GATCCAG544150.023.4796891
GATCCTG467350.023.281631
GATCCAC589500.022.568071
GATCATG704650.021.8604831
GATCCGA532350.021.6617091
GATCAGC514400.021.3646031
GATCTGC428500.021.3230151
GATCTCG459200.021.2529261
GATCCGT398250.021.2485031
GATCGTC561800.021.1774041
GATCGCA370600.020.5389041
GATCGCC283600.020.5234341
GATCGGC369600.020.131681
Produced by FastQC (version 0.11.5)