FastQCFastQC Report
Mon 9 Nov 2020
HTHGWDRXX_n01_DJ70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHGWDRXX_n01_DJ70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134070664
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGG399350.027.2225781
GATCGCG178150.023.977331
GATCCGC255850.023.5483631
GATCACG366000.023.3937911
GATCGTG429250.021.3192981
GATCCCG293600.021.0708391
GATCCAG607950.020.9847281
GATCAAC920200.020.7986031
GATCTGC437800.019.8501991
GATCGCT380450.019.657761
GATCCGT453350.019.6409681
GATCCAC608700.019.6396731
GATCAGC496250.018.9962581
GATCCGA503550.018.4283541
GATCCTG500150.017.8316251
GATCACC435150.017.8309351
GATCCCC340850.017.7595961
GATCATG732450.017.5799731
GATCCTT1305300.017.5381341
GATCGTC560200.017.4893021
Produced by FastQC (version 0.11.5)