FastQCFastQC Report
Mon 9 Nov 2020
HTHGWDRXX_n01_DJ64-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHGWDRXX_n01_DJ64-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95173200
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCG114950.026.123091
GATCACG267300.025.7386671
GATCAAC704100.024.8937741
GATCCGC171050.024.8608741
GATCGTG301050.024.0999891
GATCCGG207750.022.3444651
GATCCCG204250.021.982961
GATCCTG323100.021.6905981
GATCCAG399600.021.4262281
GATCCAC421700.021.3286551
GATCATG489150.021.000521
GATCCGA373850.020.9320931
GATCGCA253600.020.740291
GATCGTC392600.020.2591691
GATCCTT683700.019.5905191
GATCCCA339500.019.1733151
GATCGCT209300.019.1597421
GATCCGT275150.019.1504271
GATCCTC469900.019.0498661
GATCCCT240750.018.8474881
Produced by FastQC (version 0.11.5)