FastQCFastQC Report
Fri 9 Jul 2021
HTHCVAFX2_n01_7797-antiPHD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHCVAFX2_n01_7797-antiPHD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences618451
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC30810.49818013068133127TruSeq Adapter, Index 6 (100% over 50bp)
CTAATGTATGTAAGTTACTATTTGGTCTTTTTATTTTTTATTTTTTATTT7160.11577311702948173No Hit
CTTTGTGAAAGCCCTTCTCTTTTAACCCATCTTTGCAACGAAAAAAAAAA6960.11253923107893754No Hit
CTATTTGGTCTTTTTATTTTTTATTTTTTATTTTTTTTTTTTCGTTGCAA6510.10526298769021313No Hit
TGTACCATTTACTAATGTATGTAAGTTACTATTTGGTCTTTTTATTTTTT6510.10526298769021313No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)