FastQCFastQC Report
Fri 15 Jan 2021
HTHCTDRXX_l02_n01_ZmTARGET.20190625-4432-minusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHCTDRXX_l02_n01_ZmTARGET.20190625-4432-minusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8552650
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA432280.5054339882960253No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC264940.3097753327915909No Hit
GGGCGGTAAACTCCGTCCAAGGCTAAATACAGGCGAGAGACCGATAGCGA180530.21108077613371293No Hit
GCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGAAAGA110130.1287671072708459No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA101360.11851297551051429No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)