FastQCFastQC Report
Fri 15 Jan 2021
HTHCTDRXX_l02_n01_ZmTARGET.20190625-3125-minusN-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHCTDRXX_l02_n01_ZmTARGET.20190625-3125-minusN-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7482306
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA234140.31292491913589204No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC173000.23121214235290563No Hit
GGGCGGTAAACTCCGTCCAAGGCTAAATACAGGCGAGAGACCGATAGCGA129780.1734492013558387No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA104020.13902131241357946No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)