FastQCFastQC Report
Fri 15 Jan 2021
HTHCTDRXX_l01_n01_optHR_HEK_wt_GFP_BFP_R02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHCTDRXX_l01_n01_optHR_HEK_wt_GFP_BFP_R02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46201713
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAAATGATCCAGATGCAA623400.1349300620087398No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGCCTGGCGCGGATCGGT541100.11711686967104445No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAAATGATCCA526460.11394815599153217No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAAATGATCC520430.11264300957845438No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAAATGATCCAG491890.10646574944093523No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGCCTGGCGCG464060.10044216325918477No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)