FastQCFastQC Report
Fri 15 Jan 2021
HTHCTDRXX_l01_n01_optHR_HEK_wt_BFP_R03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHCTDRXX_l01_n01_optHR_HEK_wt_BFP_R03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62770685
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGCGGTGCGTGGACTGAA1061440.16909804313908636No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGCGGTGCGTG909360.14487017307521816No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGCGGTGCGT890890.14192771673592536No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGCGGTGCGTGG886040.14115506306805478No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGGCGGTGCGTGGACTGA798440.12719950403600025No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)