FastQCFastQC Report
Fri 15 Jan 2021
HTHCTDRXX_l01_n01_optHR_HEK_DNAPK_C22_GFPBFP_R08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHCTDRXX_l01_n01_optHR_HEK_DNAPK_C22_GFPBFP_R08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50563257
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACAATGGTCTTGTGGAAAGGACGAAACACCGTCCGCACTCCTGCCGGG1695990.3354194529043095No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGTCCGCACTCCT1464390.28961544150528123No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTCCGCACTC1394010.2756962432226231No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGTCCGCACTCCTGCCGG1345650.2661319859201317No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTCCGCACTCC1189760.23530129793656293No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGTCCGCACTCCTG1130620.22360505772007527No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGTCCGCACTCCTGCCG922330.18241111327144136No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)