FastQCFastQC Report
Fri 15 Jan 2021
HTHCTDRXX_l01_n01_optHR_HEK_DNAPK_C22_BFP_R09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTHCTDRXX_l01_n01_optHR_HEK_DNAPK_C22_BFP_R09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44239331
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAGGACGGCCGGACGCGG802720.1814493985001717No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAGGACGGCCGG763070.17248678557096625No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAGGACGGCCG704800.15931524823465346No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAGGACGGCC654840.14802212990065333No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGAGGACGGCCGGACGCG653120.14763333559451883No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAGGACGGCCGGA578470.13075921062187854No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGAGGACGGCCGGACGC445130.1006186101684042No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)