FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133233299
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3790190.2844776815141386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTCG139500.037.2520379
CAGGTAC86150.036.625623
CCTTATA186800.031.241582
GCAGGTA102400.031.0539572
CCCTTAT190950.030.2064061
TATATTC155650.023.1677113
GGATAGC105300.023.0793425
TTTAAAC261700.022.1691863
GACAGTA155350.022.1203677
TACTGGA295900.021.8792157
ACTGATC154000.021.7918598
ACAAGGT187000.021.7105947
TATACTG330300.021.6558555
AGGTCGT113700.021.4937636
GGTCGTT122400.019.0721517
ACAAGGC194900.018.7063988
TAACGGG80600.018.347902145
TCTAAGG93350.017.6948552
ATATAGT118050.017.5821671
GTATACG79350.017.1628041
Produced by FastQC (version 0.11.5)