FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_H12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_H12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTACAGATACACATATAGGTGCAATAGGGTAGACACACAAATTCAGA13.8461538461538463No Hit
TCTCCGGACAGCAGACTCAACACCATATGTGCATTCATCTCGATCTTCAG13.8461538461538463No Hit
GTTAAACATAAGGGATATCTCAACAAATTTGATGACTATTATAGCTATCT13.8461538461538463No Hit
CAGATAAACTATACAAGAAGCTCAAAAGAGCAATAACGTTCCATGGGGCC13.8461538461538463No Hit
TATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTGG13.8461538461538463No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCCTCAATCCGGGCCCT13.8461538461538463No Hit
TCTCAGAAAGGCCACCAGGAGATTGATCCAGTTCAGAGTAAGCGGGAGAG13.8461538461538463No Hit
ATCCATGGCTGCACAGGATGAATTGAGCAAGGCCTTATTTATGTACACTC13.8461538461538463No Hit
CCCGTATACTGGCGAGCCGGCCGACCGCCCTGGGGCAGTCACAGGATAGC13.8461538461538463No Hit
TATTTAATGTCAGTGCTCTTATTAGATAGCCTTTCTTATTCAGTCTTTGT13.8461538461538463No Hit
GATTGGAGGAGTAAGGGTGGTGGACGTGGTTAGACAGGGTCCACCTGAGG13.8461538461538463No Hit
ACACCTGGGATGCAGATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGC13.8461538461538463No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG13.8461538461538463No Hit
TGAATACACAGTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAA13.8461538461538463No Hit
ACTTAACCCAAGGGCCGTGCTGGGAGCAGATGTACCCGCCAGGAGGAGAA13.8461538461538463No Hit
GAGATACCCAAATACAGAAGAAAAGAGAATAATGGACATGATTCCAGAGA13.8461538461538463No Hit
CCTGATGTCTGCTTAAAATGGGCGCTAATGGTTGATGATTATAGGGGAAG13.8461538461538463No Hit
TGTATTCCCTCATGGTTTGGTGCATTCACTATGAGAGCAAAATCGTCGGA13.8461538461538463No Hit
CAGCAAAGGGGAGAAGTTTTATTATTTGGACAGTGTCAAATGTCCCCAGC13.8461538461538463No Hit
TGTTAACATATGTCTGATTTACGCAAGTGTTGTATTCATAAGTAATGACG13.8461538461538463No Hit
AGTCTATGACATTCCTATCTTGAGTCAGAAAGAGGCGAAGAAAAAATTGA13.8461538461538463No Hit
CTTGATGTTGGCGTACATGCTAGAAAGAGAATTGGTCCTTAAAACAAGGG13.8461538461538463No Hit
TTGCTGAAGAACTTAGATATCATCCACACATCCGTTCATAGTCTTTTGTA13.8461538461538463No Hit
CATTGCTGCTGTGCCATCTAAATCACGAGGCCTTTTTTTCTCCATTTTCT13.8461538461538463No Hit
CGTAGCGAAAGCAGGTACTGATCCAAAAAGGAACCCTTTGTGCCACAATG13.8461538461538463No Hit
TCTTTGGTCATCGTATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTT13.8461538461538463No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)