FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_H11-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_H11-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1413236
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTATATAGTACATATGATCCCCAGTAGGATACCATTGTCTTAATGCA36300.256857311871478No Hit
ATATAGTACATATGATCCCCAGTAGGATACCATTGTCTTAATGCATCAAC29600.20944838653982775No Hit
TCATAAGACAGAGCAACCTACTGTCCTCTATCCACAATTCCAAATTTTAA20880.14774602401863524No Hit
GTCAAGAGGGTGCGCGGGCCGGAAGAACACCGGGAGGCAGCCGGAACAGC18620.1317543566679592No Hit
TAGATATGCTCTGTACCTGGGTTTCCTCACTAATAAAATGGGGCTCATAA17950.12701346413479417No Hit
ATATAGTACAGGCACCATAGTCCTCTTCTAAAATCCAGCAAATTCCAAAT17270.12220181201158192No Hit
GTCTTAATGCATCAACATTCCTGCAGTCAAATGTTTGAATCCTTGTATCA14510.10267216515854394No Hit
GTATAAATATTTACCCCAAGAAAATAGTATGTTCTGTCACATAGCTGAGG14180.10033709868698505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2700.0110.209921
CTACCGT350.003581317162.079216
AACGTTC502.0203854E-457.9426427
AGTACGT1850.050.8937648
GGTGCGC5450.049.169779
CGAAAGC6150.048.29753
GCGAAAG6300.047.201022
TTGCGCA1601.0095391E-940.788555145
GTACGTA2550.036.9229289
GGATTAC1501.1249231E-633.8693921
TACTATA21800.030.6083182
CAATTTA3900.029.7131239
AAGTGCG2202.2882887E-829.6287148
ATTTACC6150.029.4413599
ATATACT10350.029.3911954
GTACTAT23600.029.2154241
TAGGGCA1504.4297645E-528.9713234
TATATAG25800.028.0710015
GCGTTAA1306.4694474E-427.895542
CTATTGA6850.027.5475751
Produced by FastQC (version 0.11.5)