FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_H10-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_H10-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503046
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATTATGTCATCTTATCAGTCAGCACATGCTGACAGAATTGGAAATGA37420.7438683539875081No Hit
TAATAGTACCTAGCACACAGGATTGGGTGCATTTAAAAGGGTAATATGTG28470.5659522190813564No Hit
AAAGCAGGCTGTCGGTCGGCTCCTCTCTCTCCCCTCAGCCTCCTCAAAGC24520.487430572949591No Hit
CCTATTACTGTTATGATCATTCAATATTTGGTATTATTGGGCAAAAATAT23880.4747080783864696No Hit
GAAAAGTTCTGTGTCCCTCTGAAATAAAACTCTTCCCCATATCGCTATGC21210.4216314213809472No Hit
GAAATAAAACTCTTCCCCATATCGCTATGCTTAGGAAACTGAAGGGTCTG17200.34191704138388934No Hit
GGGAAAACTGGTCTGAAAAGTTCTGTGTCCCTCTGAAATAAAACTCTTCC17070.33933278467575534No Hit
ATTATATACTGACACACACACACACACACACACAGTACACCAGAGCTATA16850.3349594271696823No Hit
GTTATGATCATTCAATATTTGGTATTATTGGGCAAAAATATGCTATTGCT16640.3307848586411581No Hit
CCTCAAAGCTCCCACTCTCTCTCTGCCTACGGCGTCACGGTGACGTAGAC15790.31388779554951235No Hit
GGGTTGGGGTGTTGTCAGTTCTTCAGTTATTATGTCATCTTATCAGTCAG15600.3101108049760857No Hit
GTTCTGTGTCCCTCTGAAATAAAACTCTTCCCCATATCGCTATGCTTAGG14330.28486460482739157No Hit
CTCTCTGCCTACGGCGTCACGGTGACGTAGACCTTGAGTCACGCTCCGTC14080.2798948803886722No Hit
TCGCTATGCTTAGGAAACTGAAGGGTCTGGAGATAAATTCATTACAAGGC13940.2771118347029894No Hit
CACACACACAGTACACCAGAGCTATACATTGTGCTCACTAGAACACCATC13790.2741300000397578No Hit
GCTTTAGCATCTCTGGGCCTCAATTTCATAATCTGTAAATTAGGCATAAT13000.25842567081340473No Hit
CCCCTCAGCCTCCTCAAAGCTCCCACTCTCTCTCTGCCTACGGCGTCACG12720.2528595794420391No Hit
GTTCTTCAGTTATTATGTCATCTTATCAGTCAGCACATGCTGACAGAATT12570.2498777447788075No Hit
GGGTTGGGGTTGGGGTGTTGTCAGTTCTTCAGTTATTATGTCATCTTATC12020.238944351013625No Hit
GGTCTGAAAAGTTCTGTGTCCCTCTGAAATAAAACTCTTCCCCATATCGC11970.23795040612588111No Hit
CCTATAGGGAAAGGAGACAGCGGGGCAGCCTTCAATTGAAACTGGGGAGA11970.23795040612588111No Hit
GTACAACACCATGCTGGGCTACAAGAGGCACCTGGCCCTCCATGCGGCCA10810.21489088473022347No Hit
CAGTACACCAGAGCTATACATTGTGCTCACTAGAACACCATCACACAAAC10660.21190905006699187No Hit
ATTTCATAATCTGTAAATTAGGCATAATAGTACCTAGCACACAGGATTGG10400.20674053665072378No Hit
GTGTCCAACTGGGCAGCCAGTAAACACACCTATTACTGTTATGATCATTC10230.20336112403239465No Hit
GCCTACGGCGTCACGGTGACGTAGACCTTGAGTCACGCTCCGTCTCGGAA9630.19143378537946829No Hit
GTCCTGGATGACCTCTTGGGAAGTTACCCAGCTTTCTTTCTGTGGTACAG9380.18646406094074897No Hit
TCTTATCAGTCAGCACATGCTGACAGAATTGGAAATGATGATAGTGCTGG9190.18268707036732226No Hit
GCTATACATTGTGCTCACTAGAACACCATCACACAAACACATCAAAGGGC9160.18209070343467595No Hit
GAGCTATACATTGTGCTCACTAGAACACCATCACACAAACACATCAAAGG9150.18189191445712719No Hit
GTACACCAGAGCTATACATTGTGCTCACTAGAACACCATCACACAAACAC8960.17811492388370048No Hit
GCTTAGGAAACTGAAGGGTCTGGAGATAAATTCATTACAAGGCTAGTCTT8820.17533187819801768No Hit
CTCTCACAGTGGTCGCACTGGTGCTTCTTTTCCTTGGTTCCGCTCTGGGG8770.1743379333102738No Hit
GTTGTCAGTTCTTCAGTTATTATGTCATCTTATCAGTCAGCACATGCTGA8580.17056094273684713No Hit
GGGTAGGAGCGGTGGCCACTTCCCTCAGAAACTGTCGATGCCTCTCAGAA8300.16499485136548148No Hit
GCTCCTCTCTCTCCCCTCAGCCTCCTCAAAGCTCCCACTCTCTCTCTGCC8040.1598263379492134No Hit
ATAGTACCTAGCACACAGGATTGGGTGCATTTAAAAGGGTAATATGTGTG7990.15883239306146954No Hit
GTATAAAGTCCCGCCCAAAACTGTACCACAGAAAGAAAGCTGGGTAACTT7930.1576396591961769No Hit
GTATGCGCCCCAGCTTGAAGCCCCTTAAATCGACAAACTCCTGCATGAGC7880.15664571430843302No Hit
GTATAGAAGCATCTCTCACAGTGGTCGCACTGGTGCTTCTTTTCCTTGGT7850.1560493473757867No Hit
TCTCTGGGCCTCAATTTCATAATCTGTAAATTAGGCATAATAGTACCTAG7820.15545298044314038No Hit
GTGTTGTACTTCTTCCCACACTCCTCACACCCAAAGGCCATCTTGTTGGG7720.15346509066765265No Hit
AAATAAAACTCTTCCCCATATCGCTATGCTTAGGAAACTGAAGGGTCTGG7720.15346509066765265No Hit
CTCTTTCATCAGCTCCTGTGAGTGTGTCTTCTTGGTATGTCGAGTGAGGT7610.15127841191461616No Hit
GGATTGGGTGCATTTAAAAGGGTAATATGTGTGAATCCCTTGGGACAACA7550.15008567804932352No Hit
AGCGAAAGCAGGGAAAACTGGTCTGAAAAGTTCTGTGTCCCTCTGAAATA7480.1486941552064821No Hit
CTAGAACACCATCACACAAACACATCAAAGGGCAGGATCATGGCATTTGA7300.1451159536106042No Hit
CCATATCGCTATGCTTAGGAAACTGAAGGGTCTGGAGATAAATTCATTAC7240.14392321974531155No Hit
CACACACACACAGTACACCAGAGCTATACATTGTGCTCACTAGAACACCA7130.14173654099227506No Hit
GTGCAGGAGCTCCTATAGGGAAAGGAGACAGCGGGGCAGCCTTCAATTGA7080.14074259610453121No Hit
ATAAAACTCTTCCCCATATCGCTATGCTTAGGAAACTGAAGGGTCTGGAG7000.13915228428414103No Hit
CACCTATTACTGTTATGATCATTCAATATTTGGTATTATTGGGCAAAAAT6970.1385559173514947No Hit
CCTCTCTCTCCCCTCAGCCTCCTCAAAGCTCCCACTCTCTCTCTGCCTAC6940.13795955041884836No Hit
GTACAGAGTCGGGGAGATCGGGGAGAACAGCGGGGTTTTCCAGAGCGGTC6830.13577287166581187No Hit
CTCTCTCTCTGCCTACGGCGTCACGGTGACGTAGACCTTGAGTCACGCTC6790.1349777157556168No Hit
CTCTCTCCCCTCAGCCTCCTCAAAGCTCCCACTCTCTCTCTGCCTACGGC6750.1341825598454217No Hit
GCATTTGGTCAGCGCATCTGAAAAGGAAGGTGTGAGAAGTAAAGCCCATG6660.13239345904748273No Hit
CTCAAAGATGATTATATACTGACACACACACACACACACACACAGTACAC6620.13159830313728765No Hit
AGCGAAAGCAGGCTGTCGGTCGGCTCCTCTCTCTCCCCTCAGCCTCCTCA6480.12881525745160483No Hit
GCTGTGGGGAGTGAAAGGGAAAGGGAGTGGGTTGGGGTTGGGGTGTTGTC6380.12682736767611708No Hit
CTGTAAATTAGGCATAATAGTACCTAGCACACAGGATTGGGTGCATTTAA6350.12623100074347077No Hit
GTTTTAGGATGGGGAGACCCAGACTCTGGGTGTCAGACTGAGGGAGGGAG6330.12583342278837323No Hit
CTTCAGTTATTATGTCATCTTATCAGTCAGCACATGCTGACAGAATTGGA6320.12563463381082446No Hit
GACGTAGACCTTGAGTCACGCTCCGTCTCGGAACAGTGACCAGATCTTGT6320.12563463381082446No Hit
GACAAAAGAGAGAGTGAGAGTCGGAACTAGTGGAGGGGTTGTGCCTCTCC6270.1246406889230806No Hit
GTATTATTGGGCAAAAATATGCTATTGCTACAAATAGTAACACTCTTCAC6030.11986975346191003No Hit
ATTCAATATTTGGTATTATTGGGCAAAAATATGCTATTGCTACAAATAGT6010.1194721755068125No Hit
CCCTGGAGAAGTTCACTATCCACAATTATTCCCACTCCAGGGAGCGACCT5990.11907459755171494No Hit
GTAAACACACCTATTACTGTTATGATCATTCAATATTTGGTATTATTGGG5930.11788186368642231No Hit
TCACAGCACAGTGTCCAACTGGGCAGCCAGTAAACACACCTATTACTGTT5900.117285496753776No Hit
TGTCAGTTCTTCAGTTATTATGTCATCTTATCAGTCAGCACATGCTGACA5900.117285496753776No Hit
GTGCAGGGACAGTCCGAGGTGCTTCCTGGGGAAAGAGAAAGGTGAACAAA5880.11688791879867846No Hit
GTGAAAGGGAAAGGGAGTGGGTTGGGGTTGGGGTGTTGTCAGTTCTTCAG5810.11549639595583705No Hit
CACTAGAACACCATCACACAAACACATCAAAGGGCAGGATCATGGCATTT5720.11370729515789808No Hit
ACCTAGCACACAGGATTGGGTGCATTTAAAAGGGTAATATGTGTGAATCC5710.11350850618034931No Hit
CCTCAAAGCCCATGCTGAGGAAAAGCCCCAGAGCGGAACCAAGGAAAAGA5680.11291213924770299No Hit
GGCTGGGACCATGGTTTTAGGATGGGGAGACCCAGACTCTGGGTGTCAGA5660.11251456129260544No Hit
TCTCTCTCCCCTCAGCCTCCTCAAAGCTCCCACTCTCTCTCTGCCTACGG5630.11191819435995914No Hit
TTATACTGCAGTGGAAGTGATGCTGAGACCACGGGCATCTGCGAGGGGAA5610.11152061640486159No Hit
CACACACACACACACAGTACACCAGAGCTATACATTGTGCTCACTAGAAC5570.11072546049466649No Hit
GGCTGTCGGTCGGCTCCTCTCTCTCCCCTCAGCCTCCTCAAAGCTCCCAC5570.11072546049466649No Hit
ATCCACAATTATTCCCACTCCAGGGAGCGACCTTACAAGTGCTTGCAGCC5530.1099303045844714No Hit
ATCCAGGACTGCGGACACCACCGTGAGCCATCCTGGCCCCTCGGGTTCTG5530.1099303045844714No Hit
AGGGAAAACTGGTCTGAAAAGTTCTGTGTCCCTCTGAAATAAAACTCTTC5490.1091351486742763No Hit
TCCCCATATCGCTATGCTTAGGAAACTGAAGGGTCTGGAGATAAATTCAT5470.10873757071917875No Hit
CTGTTATGATCATTCAATATTTGGTATTATTGGGCAAAAATATGCTATTG5460.10853878174163No Hit
CCCTAGCTCCAGGGCACAGACCCCACAGGTGAGGTCGCCGCTGCTGGCCG5440.10814120378653246No Hit
CACACACAGTACACCAGAGCTATACATTGTGCTCACTAGAACACCATCAC5400.10734604787633736No Hit
ATATTACCCTGCATCTAATGTACTTGAATTCCAGCAAACTGCTAAGGCCA5390.10714725889878858No Hit
ACTTTATACTGCAGTGGAAGTGATGCTGAGACCACGGGCATCTGCGAGGG5340.10615331401104472No Hit
CAATTTCATAATCTGTAAATTAGGCATAATAGTACCTAGCACACAGGATT5270.1047617911682033No Hit
GGGTAGAACCAGGCCCAGGACCACGACGCGGCCTCTCCCCTGGTCTGGAG5270.1047617911682033No Hit
TTCATAATCTGTAAATTAGGCATAATAGTACCTAGCACACAGGATTGGGT5200.10337026832536189No Hit
CATCTTATCAGTCAGCACATGCTGACAGAATTGGAAATGATGATAGTGCT5140.10217753446006925No Hit
CTGTGAGAGATGCTTCTATACCCGGAAGGACGTGCGGCGCCACCTGGTGG5140.10217753446006925No Hit
ATATTTGGTATTATTGGGCAAAAATATGCTATTGCTACAAATAGTAACAC5100.10138237854987417No Hit
CAGGGAAAACTGGTCTGAAAAGTTCTGTGTCCCTCTGAAATAAAACTCTT5060.10058722263967908No Hit
GCTCAGCACCATCCTTCCATTTGTGCTTAAATGGCACAGCATTTGGTCAG5060.10058722263967908No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAA100.0070801424145.06624145
CGATATT100.0070801424145.06624145
GAGCGAA100.007090691144.994031
AGCGAAA3600.0116.800741
CGAAAGC5300.079.2495653
GCGAAAG5750.073.061952
TAGTCCA300.001931675872.53312145
AGCAGGC5900.069.962863
AGCAGTA955.456968E-1268.5789647
TATGCGC1750.066.223652
CTGTCGG6200.063.04849
CTTATAT350.003581550462.0723153
CTATTTT400.006038216454.39984145
CCTTACC400.00607160254.3240852
GAAAGCA7950.053.7279244
AGGCTGT7400.052.8190886
GATGGGT553.241912E-452.6464777
AAGCAGG11400.051.464922
CAGGCTG8500.049.3898745
CCTTATA450.009672148.2880742
Produced by FastQC (version 0.11.5)