FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_H08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_H08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496283
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATTATGTCATCTTATCAGTCAGCACATGCTGACAGAATTGGAAATGA37010.7457438598541558No Hit
TAATAGTACCTAGCACACAGGATTGGGTGCATTTAAAAGGGTAATATGTG28200.5682241785432908No Hit
AAAGCAGGCTGTCGGTCGGCTCCTCTCTCTCCCCTCAGCCTCCTCAAAGC24350.49064747331663583No Hit
CCTATTACTGTTATGATCATTCAATATTTGGTATTATTGGGCAAAAATAT23740.47835609924176326No Hit
GAAAAGTTCTGTGTCCCTCTGAAATAAAACTCTTCCCCATATCGCTATGC21100.42516064422919986No Hit
GAAATAAAACTCTTCCCCATATCGCTATGCTTAGGAAACTGAAGGGTCTG17030.3431509844181646No Hit
GGGAAAACTGGTCTGAAAAGTTCTGTGTCCCTCTGAAATAAAACTCTTCC17010.34274798854685734No Hit
ATTATATACTGACACACACACACACACACACACAGTACACCAGAGCTATA16730.3371060463485552No Hit
GTTATGATCATTCAATATTTGGTATTATTGGGCAAAAATATGCTATTGCT16500.3324715938285212No Hit
CCTCAAAGCTCCCACTCTCTCTCTGCCTACGGCGTCACGGTGACGTAGAC15660.31554576723361466No Hit
GGGTTGGGGTGTTGTCAGTTCTTCAGTTATTATGTCATCTTATCAGTCAG15480.311918804391849No Hit
GTTCTGTGTCCCTCTGAAATAAAACTCTTCCCCATATCGCTATGCTTAGG14230.28673156243514286No Hit
CTCTCTGCCTACGGCGTCACGGTGACGTAGACCTTGAGTCACGCTCCGTC13990.2818956119794553No Hit
TCGCTATGCTTAGGAAACTGAAGGGTCTGGAGATAAATTCATTACAAGGC13830.2786716450089969No Hit
CACACACACAGTACACCAGAGCTATACATTGTGCTCACTAGAACACCATC13710.2762536697811531No Hit
GCTTTAGCATCTCTGGGCCTCAATTTCATAATCTGTAAATTAGGCATAAT12930.26053683080016843No Hit
CCCCTCAGCCTCCTCAAAGCTCCCACTCTCTCTCTGCCTACGGCGTCACG12580.2534844030522907No Hit
GTTCTTCAGTTATTATGTCATCTTATCAGTCAGCACATGCTGACAGAATT12480.2514694236957542No Hit
CCTATAGGGAAAGGAGACAGCGGGGCAGCCTTCAATTGAAACTGGGGAGA11910.23998404136349624No Hit
GGGTTGGGGTTGGGGTGTTGTCAGTTCTTCAGTTATTATGTCATCTTATC11910.23998404136349624No Hit
GGTCTGAAAAGTTCTGTGTCCCTCTGAAATAAAACTCTTCCCCATATCGC11870.23917804962088163No Hit
GTACAACACCATGCTGGGCTACAAGAGGCACCTGGCCCTCCATGCGGCCA10760.21681177876332658No Hit
CAGTACACCAGAGCTATACATTGTGCTCACTAGAACACCATCACACAAAC10530.21217732624329266No Hit
ATTTCATAATCTGTAAATTAGGCATAATAGTACCTAGCACACAGGATTGG10340.2083488654658733No Hit
GTGTCCAACTGGGCAGCCAGTAAACACACCTATTACTGTTATGATCATTC10110.20371441294583936No Hit
GCCTACGGCGTCACGGTGACGTAGACCTTGAGTCACGCTCCGTCTCGGAA9520.19182603474227408No Hit
GTCCTGGATGACCTCTTGGGAAGTTACCCAGCTTTCTTTCTGTGGTACAG9320.1877960760292011No Hit
GCTATACATTGTGCTCACTAGAACACCATCACACAAACACATCAAAGGGC9120.18376611731612807No Hit
TCTTATCAGTCAGCACATGCTGACAGAATTGGAAATGATGATAGTGCTGG9110.18356461938047444No Hit
GAGCTATACATTGTGCTCACTAGAACACCATCACACAAACACATCAAAGG9100.1833631214448208No Hit
GTACACCAGAGCTATACATTGTGCTCACTAGAACACCATCACACAAACAC8910.17953466066740145No Hit
CTCTCACAGTGGTCGCACTGGTGCTTCTTTTCCTTGGTTCCGCTCTGGGG8720.17570619988998212No Hit
GCTTAGGAAACTGAAGGGTCTGGAGATAAATTCATTACAAGGCTAGTCTT8680.17490020814736754No Hit
GTTGTCAGTTCTTCAGTTATTATGTCATCTTATCAGTCAGCACATGCTGA8540.17207923704821643No Hit
GGGTAGGAGCGGTGGCCACTTCCCTCAGAAACTGTCGATGCCTCTCAGAA8240.16603429897860697No Hit
GCTCCTCTCTCTCCCCTCAGCCTCCTCAAAGCTCCCACTCTCTCTCTGCC7990.16099685058726573No Hit
ATAGTACCTAGCACACAGGATTGGGTGCATTTAAAAGGGTAATATGTGTG7870.15857887535942194No Hit
GTATAAAGTCCCGCCCAAAACTGTACCACAGAAAGAAAGCTGGGTAACTT7860.1583773774237683No Hit
GTATAGAAGCATCTCTCACAGTGGTCGCACTGGTGCTTCTTTTCCTTGGT7840.157974381552461No Hit
GTATGCGCCCCAGCTTGAAGCCCCTTAAATCGACAAACTCCTGCATGAGC7830.15777288361680733No Hit
TCTCTGGGCCTCAATTTCATAATCTGTAAATTAGGCATAATAGTACCTAG7750.15616090013157816No Hit
AAATAAAACTCTTCCCCATATCGCTATGCTTAGGAAACTGAAGGGTCTGG7700.1551534104533099No Hit
GTGTTGTACTTCTTCCCACACTCCTCACACCCAAAGGCCATCTTGTTGGG7670.15454891664634895No Hit
CTCTTTCATCAGCTCCTGTGAGTGTGTCTTCTTGGTATGTCGAGTGAGGT7560.15233243935415883No Hit
GGATTGGGTGCATTTAAAAGGGTAATATGTGTGAATCCCTTGGGACAACA7490.1509219538045833No Hit
AGCGAAAGCAGGGAAAACTGGTCTGAAAAGTTCTGTGTCCCTCTGAAATA7430.14971296619066138No Hit
CCATATCGCTATGCTTAGGAAACTGAAGGGTCTGGAGATAAATTCATTAC7190.1448770157349738No Hit
CTAGAACACCATCACACAAACACATCAAAGGGCAGGATCATGGCATTTGA7190.1448770157349738No Hit
CACACACACACAGTACACCAGAGCTATACATTGTGCTCACTAGAACACCA7080.14266053844278367No Hit
GTGCAGGAGCTCCTATAGGGAAAGGAGACAGCGGGGCAGCCTTCAATTGA7010.1412500528932081No Hit
ATAAAACTCTTCCCCATATCGCTATGCTTAGGAAACTGAAGGGTCTGGAG6970.14044406115059352No Hit
CCTCTCTCTCCCCTCAGCCTCCTCAAAGCTCCCACTCTCTCTCTGCCTAC6930.1396380694079789No Hit
CACCTATTACTGTTATGATCATTCAATATTTGGTATTATTGGGCAAAAAT6880.13863057972971066No Hit
GTACAGAGTCGGGGAGATCGGGGAGAACAGCGGGGTTTTCCAGAGCGGTC6790.13681709830882782No Hit
CTCTCTCTCTGCCTACGGCGTCACGGTGACGTAGACCTTGAGTCACGCTC6770.13641410243752053No Hit
CTCTCTCCCCTCAGCCTCCTCAAAGCTCCCACTCTCTCTCTGCCTACGGC6730.13560811069490591No Hit
GCATTTGGTCAGCGCATCTGAAAAGGAAGGTGTGAGAAGTAAAGCCCATG6640.13379462927402308No Hit
CTCAAAGATGATTATATACTGACACACACACACACACACACACAGTACAC6590.13278713959575483No Hit
AGCGAAAGCAGGCTGTCGGTCGGCTCCTCTCTCTCCCCTCAGCCTCCTCA6450.12996616849660375No Hit
GCTGTGGGGAGTGAAAGGGAAAGGGAGTGGGTTGGGGTTGGGGTGTTGTC6350.12795118914006726No Hit
CTGTAAATTAGGCATAATAGTACCTAGCACACAGGATTGGGTGCATTTAA6300.126943699461799No Hit
CTTCAGTTATTATGTCATCTTATCAGTCAGCACATGCTGACAGAATTGGA6280.12654070359049172No Hit
GTTTTAGGATGGGGAGACCCAGACTCTGGGTGTCAGACTGAGGGAGGGAG6280.12654070359049172No Hit
GACGTAGACCTTGAGTCACGCTCCGTCTCGGAACAGTGACCAGATCTTGT6270.12633920565483805No Hit
GACAAAAGAGAGAGTGAGAGTCGGAACTAGTGGAGGGGTTGTGCCTCTCC6240.1257347118478771No Hit
GTATTATTGGGCAAAAATATGCTATTGCTACAAATAGTAACACTCTTCAC6010.12110025932784318No Hit
ATTCAATATTTGGTATTATTGGGCAAAAATATGCTATTGCTACAAATAGT5970.12029426758522858No Hit
CCCTGGAGAAGTTCACTATCCACAATTATTCCCACTCCAGGGAGCGACCT5910.11908527997130669No Hit
GTAAACACACCTATTACTGTTATGATCATTCAATATTTGGTATTATTGGG5900.11888378203565304No Hit
TCACAGCACAGTGTCCAACTGGGCAGCCAGTAAACACACCTATTACTGTT5890.1186822840999994No Hit
TGTCAGTTCTTCAGTTATTATGTCATCTTATCAGTCAGCACATGCTGACA5850.1178762923573848No Hit
GTGCAGGGACAGTCCGAGGTGCTTCCTGGGGAAAGAGAAAGGTGAACAAA5820.11727179855042386No Hit
GTGAAAGGGAAAGGGAGTGGGTTGGGGTTGGGGTGTTGTCAGTTCTTCAG5690.11465232538692641No Hit
CACTAGAACACCATCACACAAACACATCAAAGGGCAGGATCATGGCATTT5680.11445082745127277No Hit
ACCTAGCACACAGGATTGGGTGCATTTAAAAGGGTAATATGTGTGAATCC5670.11424932951561913No Hit
GGCTGGGACCATGGTTTTAGGATGGGGAGACCCAGACTCTGGGTGTCAGA5630.11344333777300451No Hit
CCTCAAAGCCCATGCTGAGGAAAAGCCCCAGAGCGGAACCAAGGAAAAGA5580.11243584809473627No Hit
TCTCTCTCCCCTCAGCCTCCTCAAAGCTCCCACTCTCTCTCTGCCTACGG5570.11223435015908262No Hit
TTATACTGCAGTGGAAGTGATGCTGAGACCACGGGCATCTGCGAGGGGAA5550.11183135428777533No Hit
GGCTGTCGGTCGGCTCCTCTCTCTCCCCTCAGCCTCCTCAAAGCTCCCAC5530.11142835841646802No Hit
CACACACACACACACAGTACACCAGAGCTATACATTGTGCTCACTAGAAC5520.11122686048081437No Hit
ATCCACAATTATTCCCACTCCAGGGAGCGACCTTACAAGTGCTTGCAGCC5490.11062236667385343No Hit
ATCCAGGACTGCGGACACCACCGTGAGCCATCCTGGCCCCTCGGGTTCTG5470.11021937080254612No Hit
CTGTTATGATCATTCAATATTTGGTATTATTGGGCAAAAATATGCTATTG5440.10961487699558518No Hit
AGGGAAAACTGGTCTGAAAAGTTCTGTGTCCCTCTGAAATAAAACTCTTC5430.10941337905993154No Hit
ATATTACCCTGCATCTAATGTACTTGAATTCCAGCAAACTGCTAAGGCCA5390.10860738731731694No Hit
TCCCCATATCGCTATGCTTAGGAAACTGAAGGGTCTGGAGATAAATTCAT5390.10860738731731694No Hit
CCCTAGCTCCAGGGCACAGACCCCACAGGTGAGGTCGCCGCTGCTGGCCG5380.10840588938166329No Hit
CACACACAGTACACCAGAGCTATACATTGTGCTCACTAGAACACCATCAC5350.10780139557470235No Hit
ACTTTATACTGCAGTGGAAGTGATGCTGAGACCACGGGCATCTGCGAGGG5280.10639091002512678No Hit
CAATTTCATAATCTGTAAATTAGGCATAATAGTACCTAGCACACAGGATT5250.10578641621816584No Hit
GGGTAGAACCAGGCCCAGGACCACGACGCGGCCTCTCCCCTGGTCTGGAG5240.1055849182825122No Hit
TTCATAATCTGTAAATTAGGCATAATAGTACCTAGCACACAGGATTGGGT5140.1035699389259757No Hit
CTGTGAGAGATGCTTCTATACCCGGAAGGACGTGCGGCGCCACCTGGTGG5110.10296544511901476No Hit
CATCTTATCAGTCAGCACATGCTGACAGAATTGGAAATGATGATAGTGCT5090.10256244924770745No Hit
ATATTTGGTATTATTGGGCAAAAATATGCTATTGCTACAAATAGTAACAC5060.10195795544074651No Hit
GCTCAGCACCATCCTTCCATTTGTGCTTAAATGGCACAGCATTTGGTCAG5030.10135346163378557No Hit
CAGGGAAAACTGGTCTGAAAAGTTCTGTGTCCCTCTGAAATAAAACTCTT5010.10095046576247826No Hit
GCATAATAGTACCTAGCACACAGGATTGGGTGCATTTAAAAGGGTAATAT5000.10074896782682462No Hit
CTCTCCCCTCAGCCTCCTCAAAGCTCCCACTCTCTCTCTGCCTACGGCGT4980.10034597195551732No Hit
CTCATAAGGTACATTATTTCCCAAAAACTATACCCCAATATTACCCTGCA4970.10014447401986368No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA550.0131.899781
AGCGAAA4350.0118.4065861
GCGAAAG5550.092.730262
CGAAAGC5800.089.92863
GCCGCGT300.001934288372.50827145
CCGTATA300.001934288372.50827145
CCCGTAA300.001936612872.486332
GAAAGCA8400.062.9369284
CCCCGTA350.003556710262.1813241
GACCCTA350.0035638362.149952145
AGCAGGC5700.058.4623533
CTGTCGG5800.056.1883779
AAGCAGC651.0888354E-555.8037531
CCGTAAC400.006068118454.331863
TATGCGC2200.052.7173352
GGTAATC858.877614E-751.2081531
AGGCTGT6700.049.721596
CGTAACA450.00967811248.2803844
AAGCAGG11650.047.9094162
TGTTGGC657.339802E-444.606972
Produced by FastQC (version 0.11.5)