FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_H08-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_H08-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1588619
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG37580.23655766423541455No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG37450.23573934341714406No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT37070.23334732871758426No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA33690.2120709874425523No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT33030.2079164355959484No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA32780.20634274171465908No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA31560.19866311557396707No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC30760.19362729515384117No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG28570.17984173675374648No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT27170.17102905101852617No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT26930.16951830489248837No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA26440.16643386488516126No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT26410.16624502161940655No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC25930.163223529367331No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC25180.15850244772346297No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG24120.15182998566679612No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA24010.1511375603590288No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG23500.14792722484119855No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA22450.1413177105397833No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG20750.13061659214701574No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG20460.1287911072447201No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC20180.127028570097676No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC20070.1263361447899087No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG19680.12388118233509733No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT19390.1220556974328017No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT18830.11853062313871357No Hit
CCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT18670.11752345905468838No Hit
GTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGA18440.11607566068390217No Hit
TTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGA18420.11594976517339903No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT18270.11500554884462542No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG17660.11116573577427942No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT17470.10996972842449951No Hit
CCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGG17340.10915140760622906No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG17320.1090255120957259No Hit
GTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTT17300.10889961658522276No Hit
CTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCA17220.10839603454321017No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT17100.1076406614801913No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT16780.10562633331214091No Hit
CATTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC16400.10323431861258112No Hit
GTTTAAGGTCTCCAACATCTTTGCAGTCATCAAAGTCTACTTTTTCTGGT16240.10222715452855592No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG16160.10172357248654336No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA16080.10121999044453076No Hit
ATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGAGCCTA16080.10121999044453076No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG16070.10115704268927918No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA15990.10065346064726659No Hit
CACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCAT15980.10059051289201502No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTCG100.0070880777145.02171145
AGTAGAA4900.096.281941
GCCCTGG259.3821884E-487.01303145
GCAGGTA3500.084.957352
CAGGTAC3550.083.679013
GGTACTG3400.083.093045
ATACCGG300.001927314772.5817641
AGGTACT4800.064.894244
TTATAGG9350.064.430881
GTAGAAA7500.062.854622
AGCGAAA1650.061.584531
AGGGTGA606.8447152E-660.361046
GACAGTA4950.059.9952167
CGAAAGC1700.055.459972
GTTACCT400.006024612654.4363251
CTCACGA400.006047954354.38314145
TTATACT9900.052.6836974
CCATGTC4250.051.129359
CCCTTAT10100.051.0228231
CCTTATA10300.050.696792
Produced by FastQC (version 0.11.5)