FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_H05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_H05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGCAACGGCCCAGATGGCTCGTCAATTGTTCATCAAAGACTACCGAT14.545454545454546No Hit
GGTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATATTGGTG14.545454545454546No Hit
ATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGACAAAGCGTCT14.545454545454546No Hit
TCACAAGCCTGTGTCACGTAGTAATTTCTAATTTTGCTGCTCAATGCCTT14.545454545454546No Hit
GACAATTAGAATCAGAGCAAAGCCTAAACATTTACAATAAAAGGAAACTT14.545454545454546No Hit
GGGATAAGAGTCAGCAAAATGGGAGTAGATGAATACTCCAGCACGGAGAG14.545454545454546No Hit
CTCTTAGAAAATAAAATATAGGCAAACTGGAGGCCAGATTTTAAGAATCA14.545454545454546No Hit
GTGTTTGGATGTTGACCCTTAGCACTTCTTCTACTTTCTTGAATGATGAT14.545454545454546No Hit
GCACCAAAACTGGACGGAATCGACCCAGGAGGGGCCAGCTTAAACGATCG14.545454545454546No Hit
CTGTGTGACTGAGTACCTTTGCCCAGTGAAGCCATCCGTCAGTAGGGATA14.545454545454546No Hit
GTACGGTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAAATACACC14.545454545454546No Hit
AATGAAGGGAGTGTACATAAATACGGCCTTGCTCAATGCATCCTGTGCAG14.545454545454546No Hit
GTGTGGGTGTGTGTGTGTGTGTGGGTCGGTGGGTGTGTATCTGGGGGTCT14.545454545454546No Hit
CTCCTGGTCCTTATGGCCCAGTATCTGCAACTGAGTTCCAGGGTATTGGA14.545454545454546No Hit
CTAGGGGGGCGGGCCCAGTAGGCTGAGCTTTTGGCCTGGCCGTGCCAGGT14.545454545454546No Hit
CAAGAAAACAAAAAAACAAACAAAAACAAAAACAACACAAAAAACACACA14.545454545454546No Hit
CCCACACACCGTAGAGATGCTGGCGAAAGCAGTCAAGCGAAGTTGTCAAA14.545454545454546No Hit
AACAAGGGTAGATAAACTAACTCAAGGTCGCCAGACTTATGATTGGACAT14.545454545454546No Hit
GGCCAAGATGGCTAATGATTTGAGAAAGATGAACACAATTACTCAAGACA14.545454545454546No Hit
CTTTTGAACGCTGTAAACAGAAAAGGAAATTAGAAAAACAGGATGTAAAG14.545454545454546No Hit
GATTGGTGGAGTAAGGATCCAGGAGATCGTTACACAGATTCCAAGTGAGG14.545454545454546No Hit
GAAAATGAGTGAAGAAAATACAATGACTGATAGAGAGTGGCTATCATATA14.545454545454546No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)