FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_H05-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_H05-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335257
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG134711.0088694535958247No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA109830.822538282892357No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA103920.7782771406553196No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA86110.6448945783470897No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC78410.5872277771245535No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT76570.5734476583908567No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC76020.5693286011606754No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG67550.5058951198158856No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG67400.5047717405712908No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG63570.476088123859302No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT57780.43272568501794034No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT56860.4258356256510919No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT55830.4181217548382072No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG55430.41512607685262093No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG53260.3988745237808153No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC52980.3967775491909048No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT50050.3748342079464852No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT47400.35498784129197597No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG42780.3203877605584543No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC41990.31447129653692135No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC39690.29724614811980016No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC38630.2893076014579965No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC36610.2741794276307857No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA35000.26212182373880083No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG33810.25320968173168157No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG32870.24616983846555385No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA31970.23942956299798468No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG31410.23523561381816382No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG31300.23441180237212764No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA31080.23276417948005515No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA30390.22759663495491878No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC29780.2230282260268997No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA29660.2221295226312238No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA29330.2196580882931151No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG29090.21786068150176333No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA28970.21696197810608747No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG28960.2168870861564478No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG28560.21389140817086147No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA28430.21291781282554595No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG28250.2115697577320321No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG26860.20115977673211974No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT25950.1943446093149109No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG25810.19329612201995572No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA25680.19232252667464017No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG25660.19217274277536084No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC24480.18333549271788127No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC24400.18273635712076403No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC24330.18221211347328642No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT24210.1813134100776105No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA23610.1768198930992311No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT23550.17637054140139313No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT23540.17629564945175347No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC22740.17030429348058088No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA22060.1652116409050842No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA21980.16461250530796695No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA21900.16401336971084968No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT21740.16281509851661513No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA21560.16146704342310134No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA21170.15854625738715467No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT21080.15787222984039775No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC21030.15749777009219948No Hit
CCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGG20210.15135663022174756No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC20010.14985879122895443No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC19780.1481362763872423No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT19470.14581462594841293No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT19340.1448410306030974No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA18980.14214492041606971No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG18780.14064708142327656No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC18620.13944881022904204No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC18330.13727694368949198No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA18080.13540464494850055No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA18050.13517996909958158No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC17430.13053666822192284No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA17370.13008731652408487No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC17240.12911372117876932No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA16680.12491977199894852No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG16680.12491977199894852No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA16420.12297258130831742No Hit
ATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG16390.12274790545939845No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATG16370.12259812156011914No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG16160.12102539061768633No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCA16140.12087560671840702No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT16020.11997690332273114No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA15520.11623230584074827No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTT15000.11233792445948607No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA14910.11166389691272915No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT14800.11084008546669293No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC14730.11031584181921533No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGA14720.11024094986957567No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA14610.10941713842353945No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG14570.1091175706249808No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT14460.10829375917894457No Hit
TCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAA14310.10717037993434972No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT13760.10305132270416856No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA13700.1026019710063306No Hit
TAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAA13560.10155348371137542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAA15700.0115.092351
GCAGGTA11400.0113.863762
CAGGTAC11750.0111.62693
GGTACTG12900.0102.21065
AGCGAAA3550.098.1204761
CCGCACG750.096.674286145
CCGCACA1150.094.57268145
AGGTACT16500.081.678614
ACTGATC16600.078.989348
GTACTGA17050.077.757276
GACAGTA15400.074.3249057
GGCGTCG300.001935279272.50572145
GGAATTA502.3055836E-672.50571145
GCGAAAG4950.071.7840962
GGTCAAA14000.068.939861
CGAAAGC5100.068.202333
GTAGAAA28000.066.088971
AACTGCG554.0604464E-665.91428145
AAATATA14650.065.7700655
TACTGAT20050.065.758977
Produced by FastQC (version 0.11.5)