FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_H04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_H04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC22.1739130434782608No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG22.1739130434782608No Hit
GGATTTGTGGCTAATTTTAGCATGGAGCTACCCAGCTTTGGAGTGTCTGG11.0869565217391304No Hit
GTTCTATTCTCAACACAAGCCAAAGGGGAATTCTTGAGGATGAACAGATG11.0869565217391304No Hit
ACCAACACTACGTCCCATTGCCCAATTAGCACATTAGACTTCTCTCCTTT11.0869565217391304No Hit
AATGAAGTGGGGGCAAGAATACTGACATCAGAGTCACAGCTGGCAATAAC11.0869565217391304No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC11.0869565217391304No Hit
ACTACGTCCCCTTGCCCAATTAGCTCATTAGCCTTCTCTCCTTTTGCAAG11.0869565217391304No Hit
GGTTTGGTGCATTCACTATGAGAGCAAAATCGTCGCATGATTGCAGCCCA11.0869565217391304No Hit
AACCCAGGGATCATTAATCAGGCCCTCCTCGTTTGCTTCTTATAGGCCCA11.0869565217391304No Hit
ATGATACTGATGTGGTGAACTTTGTAAGTATGGAGTTCTCACTCACTGAC11.0869565217391304No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA11.0869565217391304No Hit
GAGCTAATGGATGATGATTATCGGGGAAGACTTTGTAATCCCCTGAATCC11.0869565217391304No Hit
CTAATTGATGGCCCTCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA11.0869565217391304No Hit
CCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGATAGGGTTCATGTT11.0869565217391304No Hit
AATATGTTCAATTTGTAGAGCATAAAAGAACTGCGAGATCTAATGTCGCA11.0869565217391304No Hit
GAAAATATGGCACCAGAAAAAGTAGACTTTGATGACTGCAAAGATGTTGG11.0869565217391304No Hit
GGATAAAGGGAATGAAGTCGTCGTGGTTTGGCGCATTAGCCATACATCTG11.0869565217391304No Hit
GGATGAAATTTCACAGCATATTCCATAGAGTCCCATAACGGAATCAGAGG11.0869565217391304No Hit
GCAGCACTGAGTCATGGGATGATGATGGGCCTGGGCAACATGCGAAGTCA11.0869565217391304No Hit
AGAATCCACTGTTCGTTTCATTAACAACAATACGTCCCCTAGCCAAATTA11.0869565217391304No Hit
GTATTCAACAGTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGA11.0869565217391304No Hit
GATCTTATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAA11.0869565217391304No Hit
CACTGAAGTTCGCTTTCAGTACTATGTTCTTTTCCATGATCGCCTGGTCC11.0869565217391304No Hit
ACACGGAGGGCAGTCCACCTATATTACCTAGAGAAAGCCAACAAAATAAA11.0869565217391304No Hit
ATATAAATAAGACAGGGACATTTGAATTCACAAGCGTTTTTTATCGCGAT11.0869565217391304No Hit
GTTTCCGAGTTGTGTTCAAAGACTTACTTGGTCAGGTCGGTCGCTTTGAC11.0869565217391304No Hit
AGATAGAATCAGTCAAATCACATTAAGTATTTAATTCAGTTTGGACAAAG11.0869565217391304No Hit
CATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGAT11.0869565217391304No Hit
TTCCCCTTTTGGGCCTTTTGGCCAAAGCGTCTACGCAGCAGTCCTCGCTG11.0869565217391304No Hit
CCCATAGACTCCTACTGGCCATTTCTTGTCTTATAGTGAAAAGCCTAGTT11.0869565217391304No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.0869565217391304No Hit
GAACTGGGGAATTGAATCCATCGACAATGTGATGGGAATGATCGGAATAC11.0869565217391304No Hit
TCACTGGGGACAACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTC11.0869565217391304No Hit
CCTGTGAGAATACCATGGCCACAATTATTGCCTCAGCAATTGACTGCTCG11.0869565217391304No Hit
GATAAACACAGAGACTGGTGCACCCCAGCTCAACCCGATTGATGGACCAC11.0869565217391304No Hit
ATTTTTATCTCCCTGATGATGTCTGGCGTTGTTTATCCAGGGGCGCCGCT11.0869565217391304No Hit
CACTAAGACAACTGTGGACCATATGGCACTAATCAAAAAGTACACATCAG11.0869565217391304No Hit
TCATTGAAGTACTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTG11.0869565217391304No Hit
TTTTTGGCCAGTATGGAGCGCAAATTGTCGCATTGCCGCAAGTGCGTCAG11.0869565217391304No Hit
CTAGTACGGGCCGGCGGTGGGCTCATCCGGGTGCAGCGGACTGTCTCCGG11.0869565217391304No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA11.0869565217391304No Hit
ACTTATGATTGTAAATTAAACATAAATCAAACGGAAGAAACTTCATTTGC11.0869565217391304No Hit
TAGCAATGGAGTTAATCAGAATGATCAAACGTGGAATCAATGACCGAAAT11.0869565217391304No Hit
ATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGG11.0869565217391304No Hit
GTCCTGGGATGATGCTGGGCCTGTGCAACATGCGAAGTACGGTCGTGGGA11.0869565217391304No Hit
GTGCTGTGCGTGCCCATCGGCTTGGTCATTTTGTCTCGTACTCTCAGGTT11.0869565217391304No Hit
TTTTTGACACTTTCCATGCACGTGTTATCGCATTTGTGGTAAAATTCAAA11.0869565217391304No Hit
CTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCATAGTGAATG11.0869565217391304No Hit
CTCCGCTGCCTGTTCACTCGCTCCAGCCCTCTGTTCCATAGGCTTTGCCG11.0869565217391304No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG11.0869565217391304No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT11.0869565217391304No Hit
ATGCATCCTGTGCAGCCATGGATGACTTTCAGCTGATCCCAATGATAAGC11.0869565217391304No Hit
CAGCTACAGCGTCTCGACAGTGTGCCAGGCTGGGGCAGGGCGGGGGGATC11.0869565217391304No Hit
CTAAAGTTATTGCGACCAGAGACTATTCTCACAGATTACTTCGACCAAAT11.0869565217391304No Hit
GTTCAAGAGGCTATGTATATCCTTGAAGACTCCCCCCCTGGAATATTAGA11.0869565217391304No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGGTTTCGTTTCATTACC11.0869565217391304No Hit
GTGGTAAGGCTTGCATAAATGGTATGTGGTCGAGACGATTCTCTGTCGCT11.0869565217391304No Hit
TTGTTATGCTATTCTGGATTAGTCGTCCATCATAATCACTGAGTTTGAGT11.0869565217391304No Hit
AAACTAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT11.0869565217391304No Hit
TTGTAGAATCTGGTGACCCGAATGCACTATTGAAGCACCGATTTGAGATA11.0869565217391304No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT11.0869565217391304No Hit
GAGCTACAGGACATTGAAAATGAAGAGAAGATCCCAAGGACAAAGAACAT11.0869565217391304No Hit
GTCTTCATGCATTTGATTGCATCATATAGTGGTATTCCCTCCCCCTCGTG11.0869565217391304No Hit
GGTCCTGCTCACTTTCCCAATCGCGCCTGCCGCCACTCCCCTGGGTGCCA11.0869565217391304No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAATAGTACACATCAG11.0869565217391304No Hit
GTTTCCTTTGGCATATCCGCAAGCGATTTGCAGACAATGGATTGGGTGAT11.0869565217391304No Hit
GGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATC11.0869565217391304No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCGTCAATACTTAATT11.0869565217391304No Hit
GTGTAGGTGAAACGGCTGAATATTAACTTCTTTTGGCGTGCCAATATTAT11.0869565217391304No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT11.0869565217391304No Hit
CCTACGGGGAGCCCAGACTGTTCAAGCGTTTCGCAAAGGCTCATAGCTAA11.0869565217391304No Hit
GTATTTTTCCCATTTGTGTGGCTCCAGTCTCGGGTCAGTGAGTGAGAACT11.0869565217391304No Hit
GTCAACTTCTCAGTTCCTTGCGTTTCACTGACTTCTTCGGGAGACAATAG11.0869565217391304No Hit
TCTCTAGCCCTGTTGTGTTACATATACACTTCACCACTGTCGAGGGCATG11.0869565217391304No Hit
CAGTCAATGAGGAAAATTGCATCCTACTCTGTTCTGGTGGGGCAGCAGCA11.0869565217391304No Hit
TTTATGTACACTCCCTTCAATATGTATTCAGTAGCCCTGCAGTGGGACAC11.0869565217391304No Hit
GAGGGGAGGGGGTGGTTATGGGGGGGTGTGCGGTGTCGTCACTGGACGAG11.0869565217391304No Hit
CCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCTAATGGGTC11.0869565217391304No Hit
CATCTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGACCG11.0869565217391304No Hit
CTATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACCGAATCATGGC11.0869565217391304No Hit
CTCCCAGTAGGGGGCAATGAAAATAAGGCCAAACTGGCAAATGTTGTGAG11.0869565217391304No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG11.0869565217391304No Hit
AAATATACAATATACGCAATCTTCACATTACTGAACTCTGCTTAAAAAGG11.0869565217391304No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT11.0869565217391304No Hit
ATACTGATAGTAAGGGGAGGATTTGATACCGCAGGTCAGAACTTGTGCCA11.0869565217391304No Hit
GTATTATTCCCATTTGTGTGTCTCCAGTCTCGCGTCAGTGAGTGACTCCT11.0869565217391304No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA11.0869565217391304No Hit
ACTTCAATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGT11.0869565217391304No Hit
ACCTCTGGCCGTAACATGGTGGATTAGGAATGGCCCAACAACAAGTACAG11.0869565217391304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTAC50.0145.09
ACTCCTA50.0145.08
CCCATAG50.0145.01
ATAGACT50.0145.04
CAGATTT50.0145.0145
CATAGAC50.0145.03
CCATAGA50.0145.02
AGACTCC50.0145.06
GACTCCT50.0145.07
TAGACTC50.0145.05
Produced by FastQC (version 0.11.5)