FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_H03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_H03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACGAATGCAAGATCAAGGAAATTAGGGGCGATATCAACTGAGACAATA12.857142857142857No Hit
CTATAGTAAGATCACTCTTTGATCTCTAAGCCTTTAAAAAATGTGTATAC12.857142857142857No Hit
CCTTGGTCATGTCTTTCTCTTTGAAAGAAGACTCGGACTCAATCATGATC12.857142857142857No Hit
GATACAAGCCAAAGGGGAATGCACAAACAGACTGTGTTCTAGAGGCTATG12.857142857142857No Hit
GCCTTCTTCAGTCTCTTCAGCAGATTGAGAGCATGATTGAGGCCGAGTCT12.857142857142857No Hit
AAATAGGAGACATGCTATTGAGGACTGCGATAGGCCAAGTGTCGAGGACC12.857142857142857No Hit
CTATTTTCAAGTTACTTACAACTATTCGGAAATCAATCTACACTAACAAG12.857142857142857No Hit
GTGATACCCATAAACATTATGATTAAATGCCTTATTAGAAGATTGGAGGT12.857142857142857No Hit
GTATGCTAGAGTCAAGTTTTCGTTTAATTACCAACACAAAGAACCCTTGC12.857142857142857No Hit
AGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCATGTTCAACA12.857142857142857No Hit
CCAGCCATCTGTTCAATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCT12.857142857142857No Hit
GTTTGGAGGTTGCCCGTTAGCACTTCTTCTTGGTTCTTGACTGATGATCC12.857142857142857No Hit
CCTCTTGGGATTCAAGGAAGGTGAATTTGCAACGCAATCATATTCAATGC12.857142857142857No Hit
GCTGTGACTCTGATGTCAGTATTCTTTCCCCCACTTCATTTGGGAAAACA12.857142857142857No Hit
ATCTTTAACCGATTAGAGACCTGGATACTACTAAGCGCTTTCACTGAGGC12.857142857142857No Hit
GTGTGCAGGACCTAACTGGCAAAATCTGTATTAAACAGTCTATATGAGTC12.857142857142857No Hit
CACAAAAACTCAAAGATGAGCTAATCCTCAACCCTATTGCTGCCTTGCAT12.857142857142857No Hit
TTATTAAAAAATACGACACTAATAGATGGCCATACTAAAAATATTGGTCG12.857142857142857No Hit
GACCTGAGCTGAAGGCTTAACCGACTGAGCCCCCCAGGCCCCCCTCGCTG12.857142857142857No Hit
AAATAAACCAGTTTGTGAACAATGACAACATAAAATCCTTATTTTATACG12.857142857142857No Hit
GTAGAAACAAGGTAGTTTTTTACTCGAGCTATATGTTGACAAAATGACCA12.857142857142857No Hit
GTATGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGACGACTTTG12.857142857142857No Hit
AACGTCTGCCATATGATGATTATCTAATATCAATATGGTTTCTCCTACAG12.857142857142857No Hit
GGATAAGAGTCAGCAAAATGGGAGTAGATGAAAACTCCAGCACATAGAGA12.857142857142857No Hit
TTACAGAAGACAAGAGAATAATGTACATGATTCCAGAGAGGAATGAACAA12.857142857142857No Hit
GCTCTAATCTCTTGTTCTACTTCAAGCAGTAGTAGTAAGGCTTGCATAAA12.857142857142857No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA12.857142857142857No Hit
GTATGGAGTTCTCACTCACTTACCCGAGACTGGAACCACACAAATGGGAA12.857142857142857No Hit
AGACTCACTACCACTCTCTGCGTGCTGGAGTTGTGATCTAGTCACATTTT12.857142857142857No Hit
ATACAATGCTGTGGTAATGAAAGCCCATGGTCCAGCCAAAAGCATGGAAT12.857142857142857No Hit
CTGCGATAGGCCAAGTGTCGAGGCCCAGGTTCCTATATGTGAGAACGCAT12.857142857142857No Hit
TCCAAGAACTGTAAGTCGTTTGGTTGCCTTGTTCTAATTGAATTCTGGAG12.857142857142857No Hit
CTCACATTCTCAGTCAATGAGGAAAATTGCATCCTACTCTGGTCTGGTGG12.857142857142857No Hit
GACACAGCTTCTCAATGTGCCCACTGTGCACAGCCGAAAGGGAATGTCAG12.857142857142857No Hit
TTCTCTCCTTTAGCAAGATTGCTCAGAACAATGATGCTTAATGCTGGGCC12.857142857142857No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)