FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_H02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_H02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1826
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA40.21905805038335158No Hit
ATCATATTCCATGCTTTTGGCTGGACCATGGGCTGGCATTACCACAGCAT40.21905805038335158No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT30.16429353778751368No Hit
GCTAATGGGTCTGCTGACACTGCTGCTCTTCTTACTATGTTTCTAGCAGC30.16429353778751368No Hit
ATGCTAAAATTAGCCACAAATCCATAGCGATAAAAAAAGCTTGTGAATTC30.16429353778751368No Hit
CAAACAGACTGTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCC30.16429353778751368No Hit
CATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTAT30.16429353778751368No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT30.16429353778751368No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG30.16429353778751368No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG20.10952902519167579No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC20.10952902519167579No Hit
GATCAAGGCAGTTAGGGGCGATCTGAACTTTGTCAATAGGGCAAACCAGC20.10952902519167579No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT20.10952902519167579No Hit
GCTATGGATTTGTGGCTAATTTTAGCATGGAGCTACCCAGCTTTGGAGTG20.10952902519167579No Hit
CTCATAGTGAATGCACCAAACCATGAGGGAATACAAGCAGGAGTGGACAG20.10952902519167579No Hit
TACCAGAAGTGCTGCAATCTATTCGAGAAATTTTTCCCTAGCAGTTCATA20.10952902519167579No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.10952902519167579No Hit
ATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTGGT20.10952902519167579No Hit
GGCCAACACCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGT20.10952902519167579No Hit
AGCTTGAACAGTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAA20.10952902519167579No Hit
CTCCTGGAGTGTACATCTGCTCCCAGCACGTCCCTTGGGTTAAGTGCAAC20.10952902519167579No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA20.10952902519167579No Hit
CTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTGTTCGAATCT20.10952902519167579No Hit
CTCCTCTCCAGTGAATGAAAAGATGTGAATGTGTGTCTTCTCAGATTTTA20.10952902519167579No Hit
ATTCACAATGGTTGGGAGAAGAGCAACAGCTATTCTCAGAAAGGCAACCA20.10952902519167579No Hit
GCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTGGATCAATCTCCT20.10952902519167579No Hit
GATGATGACTAATTCACAAGACACAGAGATTTCTTTCACAATCACTGGGG20.10952902519167579No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC20.10952902519167579No Hit
CCGATAATCATCATCCATTAGCTCCCATTTTAAGCAGACTTCAGGAATGT20.10952902519167579No Hit
GAAATGGCCAGTAGGAGTCTATGGGATTCCTTTCGTCAGTCCGAAAGAGG20.10952902519167579No Hit
TTTTAGCATGGAGCTACCCAGCTTTGGAGTGTCTGGAGTAAATGAATCAG20.10952902519167579No Hit
GGTTAGAGATCAAAGAGGGAACGTACTATTGTCTCCCGAAGAAGTCAGTG20.10952902519167579No Hit
CTTATTAGATAGCCTCTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGT20.10952902519167579No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA20.10952902519167579No Hit
ATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC20.10952902519167579No Hit
GGATGTGATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGCAAGAATAC20.10952902519167579No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT20.10952902519167579No Hit
GTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAGAT20.10952902519167579No Hit
GAGCTACCCAGCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAG20.10952902519167579No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA20.10952902519167579No Hit
TCTCCGTGCTTGGGGTCATGTCGGGCAGTATTCCGATCATTCCCATCACA20.10952902519167579No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG20.10952902519167579No Hit
CTCTTCAATTGTTCATCAAAGACTACAGATACACATATAGGTGCCATAGG20.10952902519167579No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA20.10952902519167579No Hit
GGGCAATGAAAAGAAGGCCAAACTGGCAAATGTTGTGAGAAAGATGATGA20.10952902519167579No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC20.10952902519167579No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG20.10952902519167579No Hit
GCAATAGGGTTGAGGATTAGCTCATCTTTCAGTTTTGGTGGGTTCACTTT20.10952902519167579No Hit
AATAAGGCCTCTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGA20.10952902519167579No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC20.10952902519167579No Hit
CTATTGTTCTTTGCGTGACCATCTTCTTGGTCATGTTGTCTCTTACTCTC20.10952902519167579No Hit
GCTCAGTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGC20.10952902519167579No Hit
CTCTTCATGTTCTTTGTCCTTGGGATCTTCTCTTCATTTTCAATGTCCTG20.10952902519167579No Hit
ATCGGAGAGTCACCCAGGGGAGTGGAGGAAGGCTCTATTGGGAAAGTGTG20.10952902519167579No Hit
GCATTGTTTACAGAATCAATCTCTTTATGACTGACAAAGGGATTCAGGGG20.10952902519167579No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT20.10952902519167579No Hit
TTATTAGATAGCCTCTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTT20.10952902519167579No Hit
CCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTTGAGTTAAAGA20.10952902519167579No Hit
AATCAAAACTAGGCTTTTCACTATAAGACAAGAAATGGCCAGTAGGAGTC20.10952902519167579No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT20.10952902519167579No Hit
CAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCT20.10952902519167579No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT20.10952902519167579No Hit
GACTAGCACTGACTCAGGGCCATTGATCTCCCACATCATTGATGACGAAT20.10952902519167579No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)