FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_H01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_H01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATCAGGAATGCAAAAGAATAACCCATCACTCAGAATGAAGTGGATGA15.555555555555555No Hit
ACACTGATCCTCTCACATTCACAGTCAATGAGGAAAATTGCATCCTACTC15.555555555555555No Hit
CTCAAATGAGACCTTCCTTTTATAATGAACCCATACAGGTTTGTTTTCCG15.555555555555555No Hit
TACCATTCCATTAAAGTCCTCCGATGATTACCCAAACTGAAATTTTGACA15.555555555555555No Hit
ATCCATGGCTGCACAGGATGCAATGAGCAAGGCCGTATTTATGTACACTC15.555555555555555No Hit
CAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCT15.555555555555555No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG15.555555555555555No Hit
CTGTGTGCTGTGGCACATTTCCAAGAGAGATGCTAATGGGTCTGCTGACA15.555555555555555No Hit
ATCTGAGGGGGCGTCGTGTCGTCCTCAAGAATGGTTCAATTTTGTCGTTC15.555555555555555No Hit
GTACGGTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAAATACACC15.555555555555555No Hit
AGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGATCATGGAAAAG15.555555555555555No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT15.555555555555555No Hit
AAGAAATACAAGGTCATGTCTTTCTCGATGACAGAAGACTCTCCCAAAAT15.555555555555555No Hit
CAGATACACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAA15.555555555555555No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGGTTTCG15.555555555555555No Hit
GACTAGGGAAAGTGTACATCTTGGAGAGTAAAAGAATGAAGGTAAGCAGA15.555555555555555No Hit
GCTTCTGTTAGTCCTGCAAATTTTAAAGAAGGTCATATTTCAGACCAGCA15.555555555555555No Hit
GGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGA15.555555555555555No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)