FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_G12-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_G12-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1300844
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG34440.2647511923028434No Hit
GGATGTAATCATGGAAGTTGTTTTCCCTAACGAAGTGGGAGCCAGGATAC32730.25160588048989735No Hit
AACAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAGAG32540.2501452902884589No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG31520.24230422710178928No Hit
ATGTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAA30440.23400192490413918No Hit
CTTCATATCCCTCATGCACTCTTATCTTCAATGTTTGAAGATTGCCCGTA30360.23338693955616507No Hit
GTATTTGTTATTGGTCCATTCCTATTCCACCATGTCACAGCCAGAGGTGA29720.2284670567723724No Hit
GTATTGGCCTCTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATT28600.2198572619007352No Hit
GTATAGCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGT27780.21355366208400087No Hit
GATGTATACTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCT27030.2077881744467438No Hit
TCCTTAGGCAGAACCCAACAGAAGAGCAAGCCGTGGATATATGCAAGGCT26620.20463637453837663No Hit
ATTACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGACCAGGATTTAT26340.20248392582046734No Hit
GCCTAAGGATGTCTACCATCCTAATTCCACCAATCTGTGTGCTGTGGCAC26010.19994711126007422No Hit
ATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTACCTAA25200.19372038461183663No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA24690.18979985301850183No Hit
GTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAACT24500.18833926281706337No Hit
GATATATGCAAGGCTGCAATGGGACTGAGAATTAGCTCATCCTTCAGTTT22430.17242651693823396No Hit
GTATCAAATGTCCCAAGCACATCCCTCATTTGTTGGAACAGAGTTCTTAC22110.1699665755463376No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA22010.16919784386137002No Hit
CTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATTCCATTTTATT21380.16435483424607408No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC21250.1633554830556162No Hit
CTACAATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTA20560.15805123442933972No Hit
GTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAACACTTCAAT20500.15758999541835914No Hit
TAACAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAGA20460.15728250274437214No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT20440.15712875640737858No Hit
GTTTTATTATCTGTGCGGTATCAAATGTCCCAAGCACATCCCTCATTTGT20350.15643689789090776No Hit
GGGTAGTGGTGAGCATTGACCGTTTTTTGAGAATCCGGGACCAACGAGGA19990.1536694638250244No Hit
GGTCAATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAAAATTC19440.14944143955770256No Hit
TGTTATAGTTGAATACAGGAGAATTGCCCCTTACAAGTATTCTCATTCCT19440.14944143955770256No Hit
CTCCAACATGTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTT19290.14828834203025112No Hit
CCACTACCCTCTCCGTGCTGGAGTACTCATCTACACCCATTTTGCTGATT18970.1458284006383548No Hit
GATATGAAGAGTTCACAATGGTTGGGAGAAGAGCAACAGCCATACTCAGA18560.1426766007299876No Hit
GCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGTTTCTG18530.1424459812244973No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA18250.14029353250658805No Hit
GTGCTGGAGTACTCATCTACACCCATTTTGCTGATTCTCACTCCTCTCAT18240.14021665933809127No Hit
GTGTGTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAG18150.13952480082162041No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT18010.13844857646266578No Hit
CCCATATGTCCTTCCGAGTGAGAGACACAAAAAATTCCAACACACTATTG18000.138371703294169No Hit
GCATATATCCACGGCTTGCTCTTCTGTTGGGTTCTGCCTAAGGATGTCTA17790.13675736675573705No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA17710.13614238140776297No Hit
GTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTC17470.1342974253638407No Hit
CTTTATAGCCAACCTTTGTTCATGGCAGCCAGCATATGGGCATATGTTGC17290.13291370833089902No Hit
CTCTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCTCTC17260.13268308882540875No Hit
ATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTACCTAAGGCCA17130.13168373763495084No Hit
GAACAGAGAAACTGACAATAACTTACTCATCGTCAATGATGTGGGAGATT17060.13114562545547354No Hit
AACTTACTCATCGTCAATGATGTGGGAGATTAATGGTCCTGAATCAGTGT16930.13014627426501563No Hit
GTAGTACATTTCCTCGTTGGTCCCGGATTCTCAAAAAACGGTCAATGCTC16900.12991565475952535No Hit
GTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTTCTTCTTTCT16780.12899317673756422No Hit
GTGTTGGTCAATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAA16710.12845506455808692No Hit
GTTGAATACAGGAGAATTGCCCCTTACAAGTATTCTCATTCCTGATCCCC16700.12837819138959014No Hit
TTATTGTATAGCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTC16580.12745571336762904No Hit
ATTCTAGACGGGCCGGCCCCTGCGTGTGGCACGGGCGGCCGGGAGGGCGT16490.12676385485115815No Hit
CTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCTTGATTATT16430.1263026158401776No Hit
ATCCCAATCATTCCCATCACATTGTCGATAGGTTCAACTCCCCAATTTTG16420.12622574267168085No Hit
ATCTTATCATGTCTGGATCCGCTGCATTAATGAATCAGGGGATAACGCAG16220.12468827930174563No Hit
GAAGATAAGAGTGCATGAGGGATATGAAGAGTTCACAATGGTTGGGAGAA16000.1229970695948169No Hit
CTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCTCTCTT15930.12245895741533958No Hit
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT15750.12107524038239789No Hit
GTGTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAGAT15600.11992214285494648No Hit
GGATCAGACCGAGTGATGGTATCACCTCTGGCTGTGACATGGTGGAATAG15550.11953777701246267No Hit
CAATTATTGCTTCGGCAATCGACTGTTCGTCTCTCCCACTCACTATCAGC15470.1189227916644886No Hit
GTGGAATAGGAATGGACCAATAACAAATACAGTTCATTATCCAAAAATCT15340.11792344047403072No Hit
CTCCAGCACGGAGAGGGTAGTGGTGAGCATTGACCGTTTTTTGAGAATCC15290.11753907463154689No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT15170.11661659660958579No Hit
GTATTTTCACAAGAGGATTGTATGATAAAAGCAGTCAGAGGTGATCTGAA14890.11446414789167648No Hit
GTTTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCC14890.11446414789167648No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGA14830.11400290888069593No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT14710.1130804308587348No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT14630.11246544551076071No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG14540.11177358699428987No Hit
GTCGATAGGTTCAACTCCCCAATTTTGAAAAAGCACTTTCGCATCCTTCT14480.11131234798330929No Hit
CTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG14430.11092798214082548No Hit
CTCATTGACATCTCGATGCTTGGAGTCATGTCTGGCAATATCCCAATCAT14380.1105436162983417No Hit
AGATAAGAGTGCATGAGGGATATGAAGAGTTCACAATGGTTGGGAGAAGA14090.10831429441193563No Hit
GAATGAGGAATCCCCTCAGAACAGCGGACTCCACTCCAGCTGTGCCTTCA13870.1066230847050069No Hit
GCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTG13820.1062387188625231No Hit
GTTATAGTTGAATACAGGAGAATTGCCCCTTACAAGTATTCTCATTCCTG13780.10593122618853607No Hit
GTACTAAAGACTGAAATGGTTCAAATTCCATTTTATTGTATAGCATTGTA13740.10562373351454901No Hit
ATACAGGAGAATTGCCCCTTACAAGTATTCTCATTCCTGATCCCCTCACA13700.10531624084056197No Hit
GCATTAATGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGC13620.10470125549258788No Hit
TTCCTGATCCCCTCACATTCACAGTAAATGAGGAGAACTGCATTCTACTT13510.10385565063912353No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG13430.10324066529114945No Hit
GTATTGACCAACACTGATTCAGGACCATTAATCTCCCACATCATTGACGA13360.10270255311167212No Hit
GTGAAACACAGGGAACAGAGAAACTGACAATAACTTACTCATCGTCAATG13290.10216444093219477No Hit
ATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAAAATTCAGTGG13280.10208756776369803No Hit
GATAAAAGCAGTCAGAGGTGATCTGAATTTCGTCAATAGGGCGAATCAGC13150.10108821657324014No Hit
GTAAATGAGGAGAACTGCATTCTACTTTGCTTTGGTGGAGCGGCTGCGAA13130.10093447023624663No Hit
GTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAG13090.10062697756225958No Hit
GACAATAACTTACTCATCGTCAATGATGTGGGAGATTAATGGTCCTGAAT13060.1003963580567693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGCG750.087.01119145
AGGTGTG353.4354714E-582.86781145
AAGACGC300.001934885372.50933145
TATATAT8500.068.24672
GTATATA9050.064.158361
TTTCGCG702.3329994E-762.150856145
CCGTTCC1051.6370905E-1162.150856145
ATATGAG9800.059.1434945
AACCGGC753.7587415E-758.007465145
CTTGGCC1700.055.448315145
CGCTGGG953.137211E-853.427925145
TTAGGGG2350.052.411214
TAGGGGC2500.049.2665375
GGGCAGG1056.8495865E-848.3861051
TTAGGAA901.3227109E-648.33955145
GCCCTGG450.00963488348.33955145
TTCGATT450.00963488348.33955145
CAGGGCG1101.00157195E-746.1050454
GGTAATA6800.045.8532522
ACCTACC951.9196304E-645.795364145
Produced by FastQC (version 0.11.5)