FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_G06_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_G06_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2576
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG60.2329192546583851No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT50.19409937888198758No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT40.15527950310559005No Hit
ATCTAATAAGAGCACTGACATTAAATACGATGACCAAAGATGCAGAGAGA40.15527950310559005No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA40.15527950310559005No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG40.15527950310559005No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC40.15527950310559005No Hit
ATCTGAGGGGGCGTGGTGTCGTCCTCAAGAATGGTTCAATTTTGGCGTTC30.11645962732919254No Hit
AAATGATACTGATGTGGTGAACTTTGTAAGTATGGAGTTCTCACTCACTG30.11645962732919254No Hit
GTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTT30.11645962732919254No Hit
GTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAGATGTACACTCCAGG30.11645962732919254No Hit
CTTCAGGAATGTGAAGATTCCGTATATTGTATAAGTTTGGTCCTCCATCT30.11645962732919254No Hit
GTATTCTCACAGGAGGATTGCATGATCAAGGCAGTTAGGGGCGATCTGAA30.11645962732919254No Hit
GTCTTATAGTGAAAAGCCTAGTTTTGATTCTTGCCCTGCTCTCTTCGTCA30.11645962732919254No Hit
GTTGTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCC30.11645962732919254No Hit
CCTCAACCCTGATCCTCTCACATTCACAGTCAATGAGGAAAATTGCATCC30.11645962732919254No Hit
GGATGTAATGGAATCAATGAACAAAGAGGAAATAGAGATAACAACCCACT30.11645962732919254No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC30.11645962732919254No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA30.11645962732919254No Hit
CTGCTGAAGAGACTGAAGAAGGCAGCGCCTCATTTCCATGCCCCATTTCA30.11645962732919254No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC30.11645962732919254No Hit
AACAAGGGTAGATAAACTAACTCAAGGTCGCCAGACTTATGATTGGACAT30.11645962732919254No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT30.11645962732919254No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA30.11645962732919254No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG30.11645962732919254No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG30.11645962732919254No Hit
ATATTCACTACCACTCTCTCCGTGCTGGAGTATTCATCTACTCCCATTTT30.11645962732919254No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT30.11645962732919254No Hit
CCTATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT30.11645962732919254No Hit
GACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCA30.11645962732919254No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG30.11645962732919254No Hit
GTGGACGACAAACACAGAGACTGGTGCACCCCAGCTCAACCCGATTGATG30.11645962732919254No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTTC100.0066869557144.980416
GTTGAGC100.0066869557144.98041145
AACTTCT100.0066869557144.980417
Produced by FastQC (version 0.11.5)