FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_G05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_G05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTGGTCAGTCCGCGCTGGAATCATGTCCAGTATTCTCGTGTCTGCGG12.0408163265306123No Hit
GTACATCACCTACAAGCACGAAACTTGGCTTTATTTCTACAATTCAATAT12.0408163265306123No Hit
GTGTCCTTACGAATCCACTGTACCGGCTTCTGGTTGCCTTAGGGACAAGA12.0408163265306123No Hit
CTTTTGGAAAGATTTCCTCTTAGAATCACGTTATTACCTCATATGAACCT12.0408163265306123No Hit
TCTTTTCATTCACTGGAGAGGAGATCGCCACCAAAGCGGACTACACCCTT12.0408163265306123No Hit
CTACAGGACATTGAAAATGAAGAGAAGATCCCAAGGACAAAGAACATGAA12.0408163265306123No Hit
CCTTCTCTTCCAGGACATACTTATGAGGATGTCAAAAATGCAGTTGGGGT12.0408163265306123No Hit
GGATCAGGGTTGAGGATACTGGTAAGAGGCAATTCTCCAGTATTCAATTA12.0408163265306123No Hit
GTCTGGATTTGTGTGTCTCCCCTATGGCACATATATGTGTATCTGTAGTC12.0408163265306123No Hit
CAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCA12.0408163265306123No Hit
GTCTTACTGAGTATCAACGCCGTTGGGCGGCAGCGAGGTGGTGGTAGATG12.0408163265306123No Hit
ATTGGAGCCCATCCCACCAGTATATTGTCTTGGGGTAGTTCTTTTGTCCA12.0408163265306123No Hit
GGCCTACAACACCCTGTCAAGCTTTCAGGTAGACTGTTTCCTTTGTCATA12.0408163265306123No Hit
CTATAGTTCCAACACCTATCACCGAATCACCTGCGGCAGCAGACATTCAC12.0408163265306123No Hit
CAGTACTAACTTCTTTTACATGATCGCCTGCTCCAATCGCACGCAAAGAG12.0408163265306123No Hit
ATCATGTATTGCTAATAGAGTGAGCTTCACAGGGCATTCCATCCCCTTGT12.0408163265306123No Hit
GTCCTAGAACAAGAGGTAAAACCTAGTAAGAAGGTACATTCCCATTTTTC12.0408163265306123No Hit
ACTCCACTACCAATAGGTGAAGTTACGCCAGCAAATTTTAAACAAGGTCA12.0408163265306123No Hit
GGGAAGTCCACATATATTACCTAGAGAAAGCCAACAAAATAAAATCTGAG12.0408163265306123No Hit
TCCAGATCATGTAGAATAAATCAAATACACATAGTAATCACTAAACTTTT12.0408163265306123No Hit
AGATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGC12.0408163265306123No Hit
CACAGACTACAGATACATATAGGTGCCATAGGGGAGACACACAAATTCAG12.0408163265306123No Hit
AGGGAAAGTGCACGACAAACACACAGACAGGTGCACCCCAGCTCAACCCC12.0408163265306123No Hit
CTCTTAGAAATATCAGATCAATATCAGTGATTGTGGTCATGTCGTGAATG12.0408163265306123No Hit
ATTATGTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAAATAGTT12.0408163265306123No Hit
CCTATTGGCCATGTTTCCGATTTGTTTTCAAAGAATTCCTTGGTCTTGTC12.0408163265306123No Hit
CTCCCAACCATTGTGAATTCTTCATACCCTTCATGTACTCTTATTTTCAG12.0408163265306123No Hit
AGACTAGGGAAAGGGTACATGTTCGAGAGTAAAAGAATGAAGATTCGCAC12.0408163265306123No Hit
GATATATGAAATCATCGACAGGAAACATCCAGTATTGAGATTTACATACC12.0408163265306123No Hit
ATTTTATTTGTAATCAGTGTGGTCAGCTGCATGGGCACCTCCCAGGAGCC12.0408163265306123No Hit
GTACGCCCGAGAACGATCTGGAATACGACAACAAAGCCTTCTGTTAGACA12.0408163265306123No Hit
ATACCAACAGTGGCCATGCAGATCTCAGTGCCAATGAGGCACAGCAGGGG12.0408163265306123No Hit
ATACTTAATTTCAGAGCATCCATCAGCAGGAACTTTGACCGCTGATGGCA12.0408163265306123No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACATTC12.0408163265306123No Hit
ACATAAGCCTTCTCTCCAATTGCAAGATTGCTCAGTTAATAGATGATATA12.0408163265306123No Hit
GAACATGCCCATCATCATCCCAGGACTCAATGATGCTGTGCCATCTATTA12.0408163265306123No Hit
GAGGACCATGGGCCTCGACACTTGGCCTATAGCAGGCCTCCAGCGCATGT12.0408163265306123No Hit
GCCTCTAACTGGCTTGATCATGCAATCCTCCAGTGAGAATACCATGGCCA12.0408163265306123No Hit
GACAGTGATGAGCCAGAGCCGAGATCTCTAGCAAGCTGGGAGCAAAATGA12.0408163265306123No Hit
CCCTTATACAGGACTGGCTCCATTGAGCGAAGGGACACGAACACCAAACA12.0408163265306123No Hit
GATGGTACATGCAATGAGAACTATTGGGACTCATCCTAGCTCCAGTGCTG12.0408163265306123No Hit
TCGCAACCCATGGGAGGCAGAGTAGCGGTGTCTTATACGGTGTTGAACCT12.0408163265306123No Hit
GTCTATGGGATTCCTTACGTCAGTCCGAAAGAGGCGAAGAGACAATTGAA12.0408163265306123No Hit
AAGTAGGAGTAAGGATTTCATAAATGTTATTTGTTCGCCACGAGGAGCTG12.0408163265306123No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGATTAAAATG12.0408163265306123No Hit
CTTCCACTCATGCGCCTTGAGCTCCTGAAGTCCGTCCCAGCCCTGTGCCA12.0408163265306123No Hit
GTTATTTCTCTTTTGAGCTTCTTGTATAGTTTAACTGCTCTATCCATGTT12.0408163265306123No Hit
CAAACGCCCTGAGTCAGAGCAACTCAACACATATCAATGGATAATCACCA12.0408163265306123No Hit
CCTATATGTGTATCTGTAGTCTGTGATGAACAATTGAAGAGCCATCTGGG12.0408163265306123No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)