FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_G04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_G04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGAGGGACTCAGTAGTGAGGGGAGCTGGCTGCGGTCTGTCTGCAGG11.1363636363636365No Hit
ACGGTAACCCAGCCACTGCCACATTGTCTGGGACAGAAATTACAATTGTT11.1363636363636365No Hit
GGCCTCCACCATGCTAGAAATTCCTATATGAACTGCTAGGGAAAAATTTC11.1363636363636365No Hit
AGCGGGATGAGCGGAATTGTCCTCGCCGCTCCCAAGAGTCAAGTGGTGTG11.1363636363636365No Hit
TTATTTGGACAGTGTCAAATGTCACAAGCACATCCCGCATTTGCTGGAAC11.1363636363636365No Hit
TGATAAGCAAATGTAGGACCAAAGAAGGAAGACGGAAAACAACCCTGTAT11.1363636363636365No Hit
CAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGCCCCCCAGCTC11.1363636363636365No Hit
CGATTCCGCCTTGCATTGCTTCGTTTTGAGCCCACAGCGACATTAACTAC11.1363636363636365No Hit
CCCATGTTCCTATATGTGAGAACCAATGGAACCTCCAAGATCAAGATGAA11.1363636363636365No Hit
ACTCCATACTTACAAAGTTCACCACATCAGTATCATTTCTCAAATGAGAC11.1363636363636365No Hit
GGTGGGTCCGCAGCAAAGGGGAGATGTGTTTTTATATGGACAGTGTCAAA11.1363636363636365No Hit
GTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAAATACACCAAGAC11.1363636363636365No Hit
GTGAAAGTCCGTATGTTTGCCGTCTGAGGGCTTCACTGGGTGAGAAGAGA11.1363636363636365No Hit
CACAAAATCATAAACATAAAACATAAGTCTAATTAATAAATCGTGCACAA11.1363636363636365No Hit
CTGCTGAAGAGACTGAAGAAGGCAGCGCCTCATTTCCATGCCCCATTTCA11.1363636363636365No Hit
GACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTCACAATT11.1363636363636365No Hit
GTTTCTAGCAGCGATAATCAAACTTTGGTCAACATCATCATTTCTCACTT11.1363636363636365No Hit
CACTTGTAGTGGGGGCAAGCATACTGCCATGAGAGTCACCGCGGGCCTTA11.1363636363636365No Hit
TCTCTACCCCTGTTGTGTTACATATACTGTTCACCACTGTCCAGGCCATG11.1363636363636365No Hit
TGATGAAATGAGCAAGGCGGTATTTATGTACACTCGCTTCAGTATGTATT11.1363636363636365No Hit
AATCTCGGGTGCACACTGCTGTCATGACAGCATTTATCAATAAGGGCATA11.1363636363636365No Hit
CCCCAGCTCAACCCGATTGATGTCCCACTACCTGAGGATAATGAACCAAG11.1363636363636365No Hit
TTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCC11.1363636363636365No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG11.1363636363636365No Hit
ATATTGAAGTGTTGCACTTACCCCAAGGGACGTGCTGGGCGCAGATGTAC11.1363636363636365No Hit
ATAGTGAATGCACCTAACCATGAGGGAATACAAGCAGGAGTGGACAGATT11.1363636363636365No Hit
GAATGAGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAATGGACA11.1363636363636365No Hit
GGGGGGTGCGGGCGTGGCGCGGGCGGGGGGTCGGCGGGGGCGGGGGGCCG11.1363636363636365No Hit
GTTCCTGATTATCCATTGATAAGTGTTGACTAGCACTGACTCAGGGCCAT11.1363636363636365No Hit
CCTCGATTGCTTCATATAGCCCCCCAAGATAGAAGGTTCCAGGTTCCAGG11.1363636363636365No Hit
CCTGGAAGAATGGCTGCCAGGATTAGTTGAGAATTGCTTCATTTTTGCAG11.1363636363636365No Hit
TTACAAAGTCTTCCCCGATAATCATCATCCATTAGCTCCCATTTTAAGCA11.1363636363636365No Hit
CTTTGTAATCATACAAATCAGGAAGAAATTTAGGCTTCTCTACAACTGTT11.1363636363636365No Hit
GGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTATTCTCAG11.1363636363636365No Hit
TCTTTGCAGTCATCAAAGTCTACTTTTTCTGGTGCCATATTTTCACCGAG11.1363636363636365No Hit
TCTTAGATGCAATGCTCGTCGAGCTGGCCGTGACAGCTATGAAAGAATAT11.1363636363636365No Hit
ATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCAT11.1363636363636365No Hit
ATCAATCTCTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTC11.1363636363636365No Hit
GCTAATGGGTCTGCTGACACTTCTGCTCTTCTTACTATGTTTCTAGCAGC11.1363636363636365No Hit
GCCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTT11.1363636363636365No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT11.1363636363636365No Hit
TTAAGTCATGGATGGGAAGCAGGTGCTAGAAGAGCTACAGGACATTGAGA11.1363636363636365No Hit
GAGGATAATGAACCAAGTGGGTATGCACAAACAGACTGTGTTCTAGAGGC11.1363636363636365No Hit
GGTGATGCCATCACTCGGTCTGCGCCAGCAGCGGTTGGTGTGCTCCAGTG11.1363636363636365No Hit
CTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAATT11.1363636363636365No Hit
AAACTAGGCTTTTCACTATAAGACAAGAAATGGCCAGTAGGAGTCTATGG11.1363636363636365No Hit
GACAGTAAATACGATGCCCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAA11.1363636363636365No Hit
CTTTCAGGTAGACTGTTTCCTTTGGCATATCCGCAAGCGATTTGCAGACA11.1363636363636365No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAACCGAGAACGCT11.1363636363636365No Hit
AGTGAAGAGTGTCAAGTTTGAGAAGAGGAGAGAAAGTGATTGCAGGCGGA11.1363636363636365No Hit
CAGTAGGATTCTATGGGATTCCGGTCTTCAGTCCTAAAGCGGCGACGATA11.1363636363636365No Hit
TCAATGATGTGGGAGATCAATGGCCCTGAGTCAGTGCTAGTCAACACTTA11.1363636363636365No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC11.1363636363636365No Hit
ATTCAGAGGAGAAGATGATTTGAGGTTAAGAAGCTGTGGGAGCAAGCCCA11.1363636363636365No Hit
GTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAACCTCCATGGC11.1363636363636365No Hit
GTTCACCACATCAGTATCATTTCTCAAATGAGACCTTCCTTTTATAATGA11.1363636363636365No Hit
CAATTACCTCATGGCTTGGAAGCAGGTGCTAGCAGAGCTACAGGACATTG11.1363636363636365No Hit
GACCTGAAGAGCACACAGAATGCCATTGACGAGATTACTAACAAAGTAAA11.1363636363636365No Hit
AGTAGAAACACGGTACTTTTTTGGCCAGTATGGATAGCAAATAGTAGCAT11.1363636363636365No Hit
AAACTAACAAGTTTGCTGCAATATGCACACATTTGGAAGTTTGTTTCATG11.1363636363636365No Hit
GTTTGTGCATACCCACTTGGTTCATTATCCTCAGGTAGTGGTCCATCAAT11.1363636363636365No Hit
GTGCCGCAGGTGCTGCGGTGAAAGGAGTTGGAACAATAGCAATGGAGTTA11.1363636363636365No Hit
CCCCTGGGTGACTCTCCGATTGGCCATGTTTCCGATTTGTTTTCAAAGAA11.1363636363636365No Hit
GGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTG11.1363636363636365No Hit
GAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATAC11.1363636363636365No Hit
GTCTCCTATATGAACTGCTATCCTCAAGAATTCCCCTTTGGCTTGTGTTG11.1363636363636365No Hit
CGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTC11.1363636363636365No Hit
GTAAAATAGTTCCTCCTCATGCTTGCGATATGTTCAATCGGGGCAACATC11.1363636363636365No Hit
GAATACTGACATCAGAGTCACAGCTGGCAATAACAAAAGAGAAGAAAGAA11.1363636363636365No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT11.1363636363636365No Hit
TCTCTTCATGTTACCGGTCCTTTATCTCTGCTAGCAACTGCTTCCCAGCC11.1363636363636365No Hit
AGGTGAATGTGTGTCTTCTCAGATTTTATTTTGTTGGCTTTCTCGAGGTA11.1363636363636365No Hit
CCATAGGCGGATCCGGCCCCGGAGCTGCCTGGCTTATCTTTTCGGCGTGC11.1363636363636365No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG11.1363636363636365No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA11.1363636363636365No Hit
GATTTGTGGCTAATTTTAGCATGGAGCTACCCAGCTTTGGAGTGTCTGGA11.1363636363636365No Hit
TGTGGGATCAAACCCAATCAAAGGTAGGGCTATTAGTATCAGATGGAGGA11.1363636363636365No Hit
GATGTGAATGTGTGTCTTCTCAGATTTTATTTTGTTGGCTTTCTCTAGGT11.1363636363636365No Hit
GGGGGGCGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGCGGG11.1363636363636365No Hit
GGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCATAGTGAATGCA11.1363636363636365No Hit
TTCCAAGCCATGAGGTAATTGGGATTTATGCCTTTCTCATGTGGTTTGAC11.1363636363636365No Hit
AGCCAGTGGGGATGAGTGTGGAAGGGAACGGTGGGGCCTGGTGGGGAGAC11.1363636363636365No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCAGCA11.1363636363636365No Hit
ATGTTCCGACGTGCCTGTTAAGGTTCGAGGCGCAGGGTTGATGAGCCTGG11.1363636363636365No Hit
CTAACGTTTTCAAGGCTGGGGAAGTTCGGTGGGAGACTTTGGTCGGCAAG11.1363636363636365No Hit
ATTTCATCTTGATCTTGGAGGTTCCATTGGTTCTCACATATAGGAACATG11.1363636363636365No Hit
AGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGC11.1363636363636365No Hit
GGGGGGGGGGGGTGGGGTGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGG11.1363636363636365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCAATG50.0145.08
GATGCAA50.0145.06
CTTAGAT50.0145.02
AAGGGGG50.0145.0145
GCAATGC50.0145.09
AGATGCA50.0145.05
ATGCAAT50.0145.07
TAGATGC50.0145.04
TCTTAGA50.0145.01
TTAGATG50.0145.03
Produced by FastQC (version 0.11.5)