FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_G03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_G03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAGGACCATAGAAGTAAGACGGAAAACAAACCTGTATGGGTTCATTA13.571428571428571No Hit
AGCAGGTGCTGATCGAGAATTGAAGACTTTGGGCGACATTGGTTCAATCC13.571428571428571No Hit
GTCGATGGATTCAATTCCCCAGTTCTGGAAAAGCACTTTTGCATCTTTTT13.571428571428571No Hit
GTACGGTCGTGGGACTCTCCATCCTGAAACTAGCACAAAAGAATTTCCCC13.571428571428571No Hit
ATACCAGAAAGAGAGTAAAATAAATATCATTAGAAAACAACTACCATAAT13.571428571428571No Hit
CAAGGAATTCTTTGAAAACAAATCGGAAACATGGCCAATCGGAGAGTCAC13.571428571428571No Hit
ATATATTATATAAGTATGGAACTAAATATGATAATAATATACATACCATT13.571428571428571No Hit
ATGCAGATTAGACGTTTCGTATACTTTCTTGAAACTTTAGATAGGAGCAT13.571428571428571No Hit
ACATCATCGCTTGGAAGCACCTGCTACCAGAGCTACAGGACATTGAAAAT13.571428571428571No Hit
TTATTCTTTGATCCAAAAGAGAACAGCAATCGTGGAACAGACAGTAATCA13.571428571428571No Hit
ATCTCAGAGAACTCATCTTTCTTGCTCCCTCCAGACTCTAAGTCCACCCT13.571428571428571No Hit
CTTCAGGAATGTGAAGATTACGTATATTGTATAAGTTTGGTCCTCCATCT13.571428571428571No Hit
TACAATATACTGGGGGGATGTGCTCCAATCATCCGACGATTTTGCTCTCA13.571428571428571No Hit
TCTATGTTGTTGTCCAATCCAGTTGCTGACTCTTTATTTCTGATTAATGT13.571428571428571No Hit
CGGTGGGAGACTTTGGTCGGCAAGCTTGCGCATAGTTCATGTAATCTCAA13.571428571428571No Hit
GTCGATGGATACAATTACCCATTGATGGAAAAGCTCTTTTGCATCTTTAT13.571428571428571No Hit
CCCGGGGATGCTGCAATCACTGGCATGAAGAGTTTCTGTTGGGGGAGAGG13.571428571428571No Hit
GACTAGGGAAAGGGTACATGTTCGAGAGTAAAAGAAAGAAGATTCGAACA13.571428571428571No Hit
GGCGAGTAGTATCAGATGGAGGACCAACCTGATACAATATACGGAATCTT13.571428571428571No Hit
ATGTCAAGCTTTCAGGTAGACTTTTTCCTTTGGAATATCCGCCAGCGATT13.571428571428571No Hit
CTACCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTTGACAATAACT13.571428571428571No Hit
ATCGCATCGTTATGATAATTTATTAAAAGATCATGCATACTATGCAATCG13.571428571428571No Hit
GTCCCATTTTTGACACTTTCCATGCACGTGTTATCGCATTTGTGGTAAAA13.571428571428571No Hit
GGAGCAAAACAAACGATAGATGAAGGCACATATGGGGTGGAGTCTGATGT13.571428571428571No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCTAAAGGTTGAAACAT13.571428571428571No Hit
ATCCAGATGAAGGCACATCTGGATCTTCAGTCAATGCACCTGCATCCTTT13.571428571428571No Hit
ATACGAGAGAAGGTATCAGATCTGGCACTATCATGGTTGAATAGGAATGG13.571428571428571No Hit
TTTTTAAGGGTTAGAGATCAAAGAGGGAACGTACTATTGTCTCCCGAAGA13.571428571428571No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)