FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_G02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_G02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1277
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA50.39154267815191857No Hit
CTTCAGGAATGTGAAGATTCCGTATATTGTATAAGTTTGGTCCTCCATCT30.23492560689115116No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG30.23492560689115116No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACCTCTTAACAATA30.23492560689115116No Hit
GAATGAAAGAGAAAATAGAATCAAAACACCGTGGTGAACTCCTTGGAGAA20.15661707126076743No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT20.15661707126076743No Hit
ATCATATTCCATGCTTTTGGCTGGACCATGGGCTGGCATTACCACAGCAT20.15661707126076743No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT20.15661707126076743No Hit
GTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTCCCTCC20.15661707126076743No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT20.15661707126076743No Hit
AGAGAAAATAGAATCAAAACACCGTGGTGAACTCCTTGGAGAAGGACCTG20.15661707126076743No Hit
ATCAAAGGTAGGGCTATTAGTATCAGATGGAGGACCAAACTTATACAATA20.15661707126076743No Hit
GAACAATATTGTTAAGAGGTCTATACTACCCAAAATCTACATATTTGATG20.15661707126076743No Hit
CCTTTATTGCCCGGGCTCAGGTCTGGCCCCTGGGTCCTGCTCCCTCTCCT20.15661707126076743No Hit
GGTTTATGCTCTCACAGGTATGCCACAGTTGGCACTTGAGTGGCTCAGTC20.15661707126076743No Hit
GCTGTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCA20.15661707126076743No Hit
GGTGAACTCCTTGGAGAAGGACCTGCATGGTGGGGGTGATATTGGGAAGG20.15661707126076743No Hit
CTGATGATCCGCTTGTCCTTTTGAAAGTGAACCCACCAAAACTGAAAGAT20.15661707126076743No Hit
ATGCTGGCTGTACGGAAAGGCTGGAGCTGACCACGTGGTGGAAAGAGCCA20.15661707126076743No Hit
GTTCTGGTCTGTTCTGGTTTTGCATGTAATTTTGTTTCCATGCACATGTC20.15661707126076743No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATTGC100.0067041945141.799994
TATTGCC100.0067041945141.799995
Produced by FastQC (version 0.11.5)