FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_G02-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_G02-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1106509
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT37030.33465611215091784No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA29940.2705807182770316No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT26830.24247430432106742No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA24200.21870585779239032No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACCTCTTAACAATA22900.20695719601015447No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC19300.17442244030550136No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG15660.141526187315241No Hit
CCTTAGTACAACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCC14800.13375399567468496No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT14570.13167538628244324No Hit
GGTCTATACTACCCAAAATCTACATATTTGATGTAATACCTATCAAAATA13880.12543955810571808No Hit
TACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCTGTGGCA13050.11793848942936751No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT13000.11748661782235842No Hit
CCTTGGAGAAGGACCTGCATGGTGGGGGTGATATTGGGAAGGGAGACACG11760.10628020196853345No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT11230.10149036293423731No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG11130.10058661972021916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4450.070.106621
CGAAAGC4950.062.925433
GCGAAAG5600.055.6719172
TACATAC10100.049.4735266
CCTTATA3200.043.0486342
ACATCGA855.377746E-542.5986946
CAATTCG1953.6379788E-1240.858449
CATACAT12250.040.7959488
CTATAGG2150.040.494251
ACATACA12550.039.818957
GGTCTAC750.001478215638.6945081
TTGTACA1752.413799E-937.312561
GTCTAGA2750.036.9356651
GACAGAC3550.036.722197
GGTTATC1001.3997476E-436.2137958
TAGACCT16600.036.208894
TATAGAC17450.034.9011422
ATAGACC18050.034.5131153
CGTTACT850.002741559634.119042
AGACCTC17900.033.983765
Produced by FastQC (version 0.11.5)