FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_F12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_F12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGATGGGCCTCTTTGCCATCAGCGGGCAAAGTTCCTGCTGATGGATGC13.225806451612903No Hit
CAATTGCAGTACTGGGTATTTAGCCCAAAGATACAAATGTACTGATCAGA13.225806451612903No Hit
GTCCCCATATTTTACCTAGAGAACGCCATCAAAATAAAATCTGAGAAGCC13.225806451612903No Hit
AATCTACACAATGCTAGAAAATACTTGTAAATCACAGATCCGATAACGGG13.225806451612903No Hit
CACCTAAGAGTGTAAATGCTGGGCTCTGATTCCCTTGGTCCTGCTTAGAC13.225806451612903No Hit
TCCTTAGTGCTCCAATCTGTGTGCTGTGGGACATTTCCATGAGAGATGCT13.225806451612903No Hit
GCACACAGATTGGAGGAGTAAGGATGGTGGACATCCTTAGACAGAATCCA13.225806451612903No Hit
ATCTAGGTCCCGGGTGCCAGGAGTGTGGCCGACCCAGGGAGGGAGAGGTG13.225806451612903No Hit
CTATATATCCAGAGTTAATTTGGTGTAGTGATTTAGATGCCTTTGCCTTC13.225806451612903No Hit
GTCCTGTCCCTCCTGCTGTGGCCCCTCGCCATTCTGCCACAAACCCTACC13.225806451612903No Hit
ATTACCCACACTCCCTCCCCTTGCCAAATTAGCACTTTAGCGTTCTCTGC13.225806451612903No Hit
ACCTTAGATATGGGAAAGTCGGTTCGTTCCTATACTCTGCACACCATCCA13.225806451612903No Hit
GGTGGGGGGGGGGGGGGGGGGGCGGGCGGGGGGGGGACGGTGGGGGGGGG13.225806451612903No Hit
CCCTTGACGAAGAGAGCAGGGCAAGAATCAAAACTAGGCTTTTCACTATA13.225806451612903No Hit
GACCTGCATGGTGGGGGTGATATTGGGAAGGGAGACACGTCAGATCCCCA13.225806451612903No Hit
ATAAGGGACTGATACAAAAAGGAACACATTGTAAGACAATTCTTCAATAA13.225806451612903No Hit
CGGTGAAATTGAATAACTGCATCTCCTTTAATTTCCAAATCAGTCAGTTA13.225806451612903No Hit
GCTCAAGGCAGTTAGGGGCGATCTGAACTTTGTCAATAGGGCCAACCAGC13.225806451612903No Hit
CCATGAGCATGGATTGTCGTCCCGTTTGTTTGTGGCATCTTCCATTTCTT13.225806451612903No Hit
CTTGTCTTGGGGAATATCTCAAACCTTTCAAATGATGACACTGAGCTCAA13.225806451612903No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA13.225806451612903No Hit
CCTACAGGTCTTTAACAACAGTAGATGCCTGGGCCGAGGCTTGGGTGTTG13.225806451612903No Hit
GTATACTACCCACTGATCCTCTCACGTTCACAGTCAATGAGGTAAATTGC13.225806451612903No Hit
GGACGACAAACACAGAGACTGGTGCACCCCAGCTCAACCCGATTGATGGA13.225806451612903No Hit
ATATTTAACACCACATGGTGAGATCAGCCTACATATAGTACGTAGTTGTA13.225806451612903No Hit
ATGATGATCTCAGATAACGATTCTTTATTTATACGTCCAAAATCTCATAC13.225806451612903No Hit
CTCCGTGGAGAAGGACCTGCATGGTGGGGGTGATATTGGGATGGGAGACA13.225806451612903No Hit
ATCCTCAAGAATTCCCCTTTGGCTTGTGTTGAGAATAGAACGATTCCTCT13.225806451612903No Hit
GTGTTCTACCCCTCCACATCCTAGCTTCCCCTAAAATATCAGTTATCAGA13.225806451612903No Hit
CCTCAGTGAAAGCCCTAAGTAGTATCAAGGTCTCTAATCCGTTAAAGATT13.225806451612903No Hit
GCTATTTACAGCCAAAGAATCTCTTCATTCATATGATTGCATCAGATAGT13.225806451612903No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)