FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_F10-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_F10-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1533454
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA130070.8482158577955387No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG101250.6602741262535426No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC79960.5214372260269953No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT78410.51132932582262No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA77410.5048080998843135No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG76530.4990694210586037No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA69770.4549859337156511No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG64360.4197061013894124No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG61770.4028161262091983No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG58860.3838393587287261No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT56720.36988393522075No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC56460.3681884164767903No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT47910.31243193470426894No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG45640.297628751824313No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC45320.2955419595240549No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT44510.2902597665140265No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT43230.28191259731299406No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC41500.27063087643972367No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG36410.2374378364137431No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC35920.23424243570397285No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG35470.23130788403173488No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG35320.23032970014098889No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT34460.22472144583404524No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG33850.22074349801167822No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG31660.20646201320678675No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG30470.19870175434020193No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG29690.19361519810832278No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA29550.19270222647695986No Hit
CCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGG28800.18781130702322993No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC28610.18657227409495167No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA28550.18618100053865325No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG27450.179007652006516No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC25170.16413925686717698No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG25010.1630958607170479No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA24090.15709633285380586No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC23480.15311838503143882No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA23290.15187935210316056No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT23010.15005340884043475No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA22520.1468580081306645No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA22280.14529291390547092No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC22020.1435973951615112No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC21900.14281484804891442No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG21470.1400107208954426No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC21370.1393585983016119No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA21060.13733701826073688No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA20950.13661968340752315No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT20720.13511980144171262No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC20590.13427204206973278No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT20490.13361991947590213No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG19740.12872900002217216No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA19360.12625093416561567No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC19030.12409892960597449No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG18890.12318595797461156No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA18730.1221425618244825No Hit
CCATGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGA18730.1221425618244825No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA18710.12201213730571638No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG18650.12162086374941797No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG18290.1192732224116276No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT18190.11862109981779696No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT18170.11849067529903082No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG18000.1173820668895187No Hit
CACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGT17710.11549091136740978No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA17470.11392581714221621No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA17340.11307805777023634No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA17300.11281720873270408No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG17170.11196944936072421No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC16880.11007829383861531No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC16770.10936095898540159No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCA16720.10903489768848626No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT16710.10896968542910318No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA16620.10838277509465559No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG16440.10720895442576041No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC16430.10714374216637736No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA16060.1047308885692039No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA15940.1039483414566071No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT15660.10212239819388128No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA15460.10081815300621993No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA15430.10062251622807075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA11350.0107.9536062
AGTAGAA18950.0106.437321
CAGGTAC12200.099.763073
GGTACTG12250.099.323475
GGTCGTG301.6084161E-596.561347
AGCGAAA3050.088.015711
ATCCGGA259.3602407E-487.064191
CCCTTAC1950.074.413841
GTAGAAA27650.072.632492
CTTACAC2000.072.446993
AGGTACT17550.072.217034
GACAGTA17150.070.5207447
ACTGATC17250.069.69218
TATATTC22350.065.801973
GCGAAAG4100.065.428082
GTACTGA19150.063.909936
CGAAAGC4450.060.2368243
ATATTCA24500.060.007934
CCGCACA851.303124E-859.722622145
TAGAAAC35050.059.1150933
Produced by FastQC (version 0.11.5)