FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_F09-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_F09-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1682176
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT40140.23861950235884952No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA38950.231545331760767No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG35790.21276013924821183No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA34640.20592375589712372No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT34590.2056265218383808No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG30920.1838095419266474No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA30740.18273949931517272No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA30080.17881600973976564No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC30000.17834043524577692No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT27790.165202689849338No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC27560.16383541317912037No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT26690.15866354055699283No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA26630.1583068596865013No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC26440.15717737026327805No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT26350.1566423489575407No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG26210.15581009359306042No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG25830.15355111474661393No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT25180.1496870719829554No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG23160.13767881600973977No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC23030.13690600745700807No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG22430.13333919875209252No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT22070.1311991135291432No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG21580.1282862197534622No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC21260.12638392177750724No Hit
CCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT21100.12543277278952977No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT20310.12073647466139097No Hit
TTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGA20060.11925030436767615No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG19710.11716966595647542No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG19630.11669409146248669No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA19550.11621851696849794No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT19410.11538626160401766No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA19390.11526736798052047No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTT18540.11021438898189012No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG18350.10908489955866686No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA17970.10682592071222037No Hit
GTGTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAG17920.1065286866534774No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT17900.10640979302998022No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG17860.10617200578298584No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT17410.10349689925429918No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG17000.1010595799726069No Hit
GTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTT16870.10028677141987521No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAA7500.098.695081
GCAGGTA3700.086.2402952
CAGGTAC3850.082.830993
GGTACTG3800.081.994085
AGCGAAA1450.080.077151
GTAGAAA9950.074.342362
GGCTCGC300.001935656172.50299145
CGAAAGC1700.068.213753
AGCGAGC651.398239E-766.92584145
AGGTACT5100.066.776754
GACAGTA5950.065.756047
GCGAAAG1800.064.462442
GCTATAC1251.8189894E-1257.9816933
TATATTC4500.054.760493
TGCGTGT400.00605061354.377243145
GCCCTGG553.2189788E-452.729446145
TTATACT14950.052.34564
CCCTTAT14950.051.4542581
CCTTATA14900.051.1048742
CTGTAGG604.9450446E-448.335327145
Produced by FastQC (version 0.11.5)