FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_F06_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_F06_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2087
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG140.6708193579300431No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG110.5270723526593196No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA110.5270723526593196No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA100.4791566842357451No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG100.4791566842357451No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA100.4791566842357451No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA90.43124101581217056No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC90.43124101581217056No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT80.3833253473885961No Hit
GAATGAATGAGATACCCAATTACAGCAGACAAGAGAATAATGGACATGAT80.3833253473885961No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT80.3833253473885961No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT70.33540967896502155No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG60.287494010541447No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC60.287494010541447No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG60.287494010541447No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC60.287494010541447No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG50.23957834211787254No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA50.23957834211787254No Hit
GAATAATGGACATGATTCCAGAGACAACAAGGCAACCAAACGACTTACAG50.23957834211787254No Hit
GTTCATTGATGCTTAATGCTGGGCCTCTTGTCTTCTTTGCCCAAAATGAG50.23957834211787254No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT40.19166267369429804No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT40.19166267369429804No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC40.19166267369429804No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA40.19166267369429804No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC40.19166267369429804No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG40.19166267369429804No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG40.19166267369429804No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA40.19166267369429804No Hit
GCTTAATGCTGGGCCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC40.19166267369429804No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG40.19166267369429804No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG40.19166267369429804No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC40.19166267369429804No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG40.19166267369429804No Hit
GGATGCAGGTGCATTGACTGAAGATCCAGATGAAGGCACATCTGGGGTGG40.19166267369429804No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC40.19166267369429804No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC30.1437470052707235No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG30.1437470052707235No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA30.1437470052707235No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG30.1437470052707235No Hit
GCTCAGTTCATTGATGCTTAATGCTGGGCCTCTTGTCTTCTTTGCCCAAA30.1437470052707235No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA30.1437470052707235No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC30.1437470052707235No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG30.1437470052707235No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG30.1437470052707235No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG30.1437470052707235No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG30.1437470052707235No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG30.1437470052707235No Hit
CTTAATGCTGGGCCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCA30.1437470052707235No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT30.1437470052707235No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA30.1437470052707235No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC30.1437470052707235No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA30.1437470052707235No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT30.1437470052707235No Hit
CAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG30.1437470052707235No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA30.1437470052707235No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT30.1437470052707235No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTAAG300.001670809372.53
TCAGTAA300.001670809372.52
AGTAAGT300.001670809372.54
GTAAGTA300.001670809372.55
GTCAGTA300.001670809372.51
Produced by FastQC (version 0.11.5)