FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_F06-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_F06-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1091135
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG132981.2187309544648461No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA109060.9995096848694249No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA107840.9883286669385548No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA101160.9271080113826429No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA89460.819880216471839No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT79580.7293323007693823No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG76220.6985386776155105No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG71380.6541811966438617No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC67710.6205464951632933No Hit
GCTTAATGCTGGGCCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC65090.5965348009183098No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT57900.5306401132765424No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG54980.5038789883928203No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT54530.49975484243471247No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC52400.4802338848996687No Hit
GAATAATGGACATGATTCCAGAGACAACAAGGCAACCAAACGACTTACAG52110.4775761019488881No Hit
GTTCATTGATGCTTAATGCTGGGCCTCTTGTCTTCTTTGCCCAAAATGAG51710.47391019443057003No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG50120.4593382120452556No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT49650.45503077071123194No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT45400.4160805033291023No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC43900.4023333501354095No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC43680.4003171010003345No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA39340.36054200442658335No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA37030.3393713885082964No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA36980.33891315006850664No Hit
GAATGAATGAGATACCCAATTACAGCAGACAAGAGAATAATGGACATGAT36960.3387298546925907No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA36110.33093980121616484No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT35480.32516599687481385No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC35290.32342469080361275No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG34690.31792582952613563No Hit
GAATGAATGAGATACCCAATTACAGCAGACAAGAGAATAAGGCAACCAAA34160.31306850206436415No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC33610.3080278792266768No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG32260.2956554413523533No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG29990.27485141618589815No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC29680.27201033785920165No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA28970.26550335201418707No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT28850.26440357975869166No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG28440.26064602455241564No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG28380.2600961384246679No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG28260.2589963661691725No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG27960.2562469355304339No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA27890.2556054017147283No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG27310.250289835813167No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT26780.24543250835139555No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA26550.24332461152836268No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG26450.24240813464878314No Hit
CTTATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCA25730.2358095011158106No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA25470.23342666122890388No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG25340.23223524128545048No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC25050.22957745833466986No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT24400.22362035861740298No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG24270.22242893867394958No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG23760.21775490658809404No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG23580.21610524820485094No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT23080.21152286380695332No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA22870.2095982623598363No Hit
TGCTTAATGCTGGGCCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCT22850.2094149669839204No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC22810.20904837623208858No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC22570.20684883172109775No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC22420.20547411640172847No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC22390.20519917333785462No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT22230.20373281033052737No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT21400.1961260522300174No Hit
CTTAATGCTGGGCCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCA21300.19520957535043784No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA20770.1903522478886664No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA20480.18769446493788577No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA19970.18302043285203023No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT19480.17852969614209058No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT19290.17678839007088948No Hit
GAATAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAGGTGCAT18800.17229765336094985No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA18210.16689043977143067No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTT18140.166248905955725No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC17870.1637744183808603No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA17580.16111663543007967No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTAAGAGGAATCG17290.15845885247929908No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA17130.15699248947197186No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA17070.15644260334422413No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC16690.15295999120182197No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG16670.15277669582590606No Hit
GCTTATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGC16520.15140198050653678No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC16280.14920243599554595No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA16260.14901914061963No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC16090.14746112992434485No Hit
GTTCATTGATGCTTATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTC15900.14571982385314375No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA15420.14132073483116206No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT14910.1366467027453065No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT14720.1349053966741054No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA14720.1349053966741054No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA14600.13380562441860996No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC14540.13325573829086226No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG14250.13059795534008165No Hit
CCCAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGACAACAA14010.12839841082909081No Hit
GGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGA13610.12473250331077274No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA13460.12335778799140344No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT13370.12253295879978189No Hit
ACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCA13370.12253295879978189No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG13370.12253295879978189No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT13170.12070000504062284No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA12940.11859210821758995No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT12900.11822551746575813No Hit
GGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAAT12800.11730904058617861No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGA12750.11685080214638886No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG12690.11630091601864115No Hit
GCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACT12680.1162092683306832No Hit
GCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACAC12620.11565938220293548No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA12460.11419301919560826No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCTCTTGTCTTC12380.11345983769194463No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT12300.11272665618828101No Hit
GGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCG12180.11162688393278558No Hit
CTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCAGACTCCA12090.11080205474116403No Hit
GAGAATAATGGACATGATTCCAGAGACAACAAGGCAACCAAACGACTTAC12010.1100688732375004No Hit
CACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGT11960.10961063479771065No Hit
CAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG11850.10860251023017317No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA11760.10777768103855159No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG11760.10777768103855159No Hit
TGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTC11620.10649461340714027No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG11420.10466165964798124No Hit
GAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATAC11310.10365353508044377No Hit
GAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAA11070.1014539905694529No Hit
GCTCAGTTCATTGATGCTTAATGCTGGGCCTCTTGTCTTCTTTGCCCAAA11030.1010873998176211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAA18250.0114.096241
GCAGGTA5600.0112.622422
CAGGTAC5800.0109.923223
CGATCCG203.879027E-4108.659155
AGCGAAA3550.0102.18661
GGTACTG6250.0101.994735
AGGGTGA406.148348E-790.549296
CCGCACG703.092282E-1182.87149145
GGTCAAA14250.078.916741
TAGTTCG950.076.252045
AGGTACT8500.075.844874
CCGCACA1350.075.1982145
ACTGATC8650.073.692238
AAATATA15400.073.380215
GCGAAAG4950.073.2248462
CGAAAGC4850.073.19633
TTCACGG300.00193446172.51255145
GTAGAAA28850.072.1156852
TCAAATA15900.070.626773
GTACTGA9050.070.438356
Produced by FastQC (version 0.11.5)