FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_F05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_F05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGATGATGTTCTGAGACATGTCATCTAACTAGGTGCCTGGTCACTGGA12.272727272727273No Hit
AGATTAAAGCTAGCCAGCCCACAAAATAAACTCTTCGCAGACACCCATTC12.272727272727273No Hit
CTGCGGCGCGGCTGACTAGGGTGCTAGCCGCGATCCTCCAACTGTGGTCA12.272727272727273No Hit
ACAATGACCTTGGACCTGCAACGGCCCAGATGGCTCTTCAATTGTTCATC12.272727272727273No Hit
GTATCACCTCTGGCCGTAACATGGTGGAATAGGAATGGCCCAACACCAAG12.272727272727273No Hit
GGAGAAAATGATGGGCTAGGGTTTCCATTGCCTGGGACTCAGGGACGNNN12.272727272727273No Hit
GAGGCTGTTGTGGCGGGTGGGGTAGGGCGCGGCGGGGGTCGGGCGGGGAG12.272727272727273No Hit
GACCTGGATCATTTGCAACTTGTGTAATAAGTATTTGTGACTTGATGATT12.272727272727273No Hit
GAATGGATCTTGAAAGAGGAATCCAGCGAGACACTTAGAATGACAATTGC12.272727272727273No Hit
AATTAGAGGATAAGAATACTCTGTTGAAAAGATATTTAGCAGCATAATTG12.272727272727273No Hit
CTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCAGACTC12.272727272727273No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC12.272727272727273No Hit
GGCTGGAGGGATGGGGCGGGTGTGCGGGCGTAGTGCCTGGCGCTCGCGAG12.272727272727273No Hit
GTCTTGTGAATTAGTCATCATCTTTCTCACAACATTTGCCAGTTTGGCCA12.272727272727273No Hit
GGATACACCATTGACACAGTAAAAAGAACACACAAAAACTCAGAAAAGCT12.272727272727273No Hit
GAATAGGAATCGCCCAACAACAAGTACAGTTGATTACCCTAAGGTATATA12.272727272727273No Hit
GGTCCAAGGTCATTGTTTATCATGTTGTTCTTTATCACTGTTACTCCAAT12.272727272727273No Hit
CGCAGGGTGACAAAAACATAATCGACTCCAACACCATGTCAAGCTTTCAG12.272727272727273No Hit
CAATTTTACAATCCTGGAGCTCTTCTTTCTTCTCTTTTGTTATTGCCAGC12.272727272727273No Hit
CCTCTAAAGTTCGTCCCAGCTGACCCGAGAGGGGGGCCAAATAACTGGCC12.272727272727273No Hit
CCCTAGCCCAATTAGCAATGGGGAAATTTCTCCAACTATTGCTCCCTCCT12.272727272727273No Hit
CGACTACGACCAATCCACTATTCCGGCAGGCACACAGATTGGTGCTGGCT12.272727272727273No Hit
GATTTTCAGTCTTCGTAGTTTATAAAAAAAAGGCCAATATTTGAGGTTGA12.272727272727273No Hit
GCATTTCGGTGTATATAGAATACAAATATTCATATAGGCAGTGGAGCAAT12.272727272727273No Hit
CTGCATGTTCCGACGGCTACTGGGAGAAACCTTGTTGTACGGACCAATTC12.272727272727273No Hit
AGTCCACACTTATCAATGGATAATCAGGAACTGGGAAATTGTGAAAATTC12.272727272727273No Hit
GCTTAATGCTGGGCCATATCTCATCACTTATTTGCACAAAATTAAAAATC12.272727272727273No Hit
GTCATTATATTAAACTTTTTGACCGAAAAGATGGTCCGTTGTGCGAGCAG12.272727272727273No Hit
GTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGAACAGTCTGGGCT12.272727272727273No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA12.272727272727273No Hit
CAATAGAGCCTTCCTCAACTACCCTGGGTGACTCTCCGATTGGCCATGTT12.272727272727273No Hit
GGTCAAATATATTCAAAATAGAGAGAAAAAAAGAAATGACACATATAATT12.272727272727273No Hit
GATGATTGGAGACCATCCACACAGTATATTGTCTTTGGGTATTTCTTTTG12.272727272727273No Hit
GAAATGCATGGCTATAAGATTAGTGTTTGCTGATTGATCCAAGATACTGC12.272727272727273No Hit
CTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCGACCAATACTTA12.272727272727273No Hit
ACCTCCAGGTCGAAAGACGGATTATTGGACATTAAACAGACATCCCCAGG12.272727272727273No Hit
CTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAAT12.272727272727273No Hit
NNCCACGAACCTATCATTTGGTGATGTGCGAAACTCATACTACTATGTTG12.272727272727273No Hit
CACTTGGAAATGTGAGCCAGCTCACAGGTTGGAGGACTATGGATGTTCTA12.272727272727273No Hit
CTGTAATACCAAGACCCCCCATTTGTCCTCAGCCGTCCTGAGAGATGCTT12.272727272727273No Hit
CTATGCTAGAGTCCAGTTTTCCTTTAATAAACACAAATACCGACCATAAG12.272727272727273No Hit
ACACAACCCAAAGGGGAATTCTTGAGGAAGAACAGATGAACCAGAACTGC12.272727272727273No Hit
CATTAACCTTCTCTCGTTTTGCAAGAATGCTCAGTTCATTGATGCTGATC12.272727272727273No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT12.272727272727273No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)