FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_F03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_F03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTTCAAGTGTTCATCAAAGACTACAGATACACATATAGGTGCCATAG13.225806451612903No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTAAG13.225806451612903No Hit
GCTGGGGAAACAATACTGAAAAAATACAACCAGCAAGCAATAAAAAGAGA13.225806451612903No Hit
GAATTAGCCACTTAGCCTTCTCTCCTTTTGCAAGCTTGCTCAGTTCAAAG13.225806451612903No Hit
TGTTCCTGGCGATGATTACATATATCACCAGAAATCAACCCGAGTGGTTC13.225806451612903No Hit
CCTGAGCATGCCACCTATAATGTTATCAAACAAAATGGAATGACTAGGGA13.225806451612903No Hit
TCCCAGGACTCAGTCATGCTGTGACATCTATTAGAAGCGGCCTTATTATC13.225806451612903No Hit
GGCCTGGACAGTGGTGAACAGTATATGTAACACAACAGGGGTAGAGAAGC13.225806451612903No Hit
CAGTAAACAGAACACACCAATACTCAGCAAAGGGAAAGTGCACCACAAAC13.225806451612903No Hit
GGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGCGGGGGG13.225806451612903No Hit
GCAAATAGTAGCATTGCCAAAACTACTTCAGTTCATGTGTGAGGAAGGAG13.225806451612903No Hit
CCTCAATAAATCCATGGAACAGGAAAAGGAGACACCATGGACACAGTAAA13.225806451612903No Hit
CATGTGACCATTGAATTATCACAATTTACCATTTACTGATTATACATTTA13.225806451612903No Hit
CAGCAATGGAACAGGAACAGGATACACAATGGACACAGGAAACAGAACAC13.225806451612903No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT13.225806451612903No Hit
CCTTCCTGACTCATTAGCTGTTAGGCCATTCGATCTAAAGACATCTATGT13.225806451612903No Hit
GTAGAGCAGCCTAAATTTATTCCTGATTTGTATATGACCAAGGAATTCTT13.225806451612903No Hit
TATCTGTAGTCTTTGATGAACAATTGAAGGGCCATCTGGGCCGTTGCAGG13.225806451612903No Hit
TATAAATGCTCTTGAGGACTGCGATAGGCCAAGTGTCGAGGACCATGTTC13.225806451612903No Hit
GGCTAAATAAAGTAAAGACAACAGACTAAGAATAGGTTCCAAGGGGCATG13.225806451612903No Hit
AAAAAATGTAATTTGAAACATTTCAGTCTATTGTCAATAAGGCAAAAAGA13.225806451612903No Hit
TGCTAGCTGTCAGTAAGTATGATAGATTCCAGTTTTTCTTTAATGAAAAA13.225806451612903No Hit
AAACAGGGTCGTTTTTAACCAAGTAGCCACTAATTGATGGCAATCCGCTT13.225806451612903No Hit
GTATTTATGTACACTCCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGA13.225806451612903No Hit
GTTACAATATGTGCGAACCAATGGAACCTCAAAGATCAAGATGAAAACGG13.225806451612903No Hit
GACTAGTTTTGATTCATGCACTCCTCTCTTCGACAAGGGTGTAGTCCGCT13.225806451612903No Hit
GTATGATAGAGTACAGGTTTCCGTTGATTAACAAAACTTCCAAACATTTA13.225806451612903No Hit
GTTCCCGCTGACTGCTGCCATAACGGTTGCTCTTTCAAAAGGGAGATTCC13.225806451612903No Hit
GCTAATGGTTCTGCTGACACTGCTGCTCTTCTTAGTATGTTACTATCCGC13.225806451612903No Hit
ATTCAATATGGACAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTACC13.225806451612903No Hit
CCCCTATGGCACCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAG13.225806451612903No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)