FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_F02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_F02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1957
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGTTCCTGATT40.2043944813490036No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT40.2043944813490036No Hit
GGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTATTCTCAG40.2043944813490036No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA30.1532958610117527No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA30.1532958610117527No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG30.1532958610117527No Hit
CTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTGTTCGAATCT30.1532958610117527No Hit
GGATGTGATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGCAAGAATAC30.1532958610117527No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG30.1532958610117527No Hit
TTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGA30.1532958610117527No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT30.1532958610117527No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC30.1532958610117527No Hit
ATACAAACCCTGGCCATGCAGATCTCAGTGCCAAGGAGGCACAGGATGTG20.1021972406745018No Hit
GTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGGCCGTTGCAG20.1021972406745018No Hit
TTCCATTACATCCTTTAAGAAATCTATTAGCCTTCCTGACTCATTAGCTG20.1021972406745018No Hit
CCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCATCATATTCCATGC20.1021972406745018No Hit
GAATGCACCAAACCATGAGGGAATACAAGCAGGAGTGGACAGATTCTACA20.1021972406745018No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT20.1021972406745018No Hit
GACTACAGATACACATATAGGTGCCATAGGGGAGACACACAAATTCAGAC20.1021972406745018No Hit
GCCTAACAGCTAATGAGTCAGGAAGGCTAATAGATTTCTTAAAGGATGTA20.1021972406745018No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT20.1021972406745018No Hit
ATATAAATAAGACAGGAACATTTGAATTCACAAGCTTTTTTTATCGCTAT20.1021972406745018No Hit
GTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGGTTTGAT20.1021972406745018No Hit
TTCCAGAACTGGGGAATTGAATCCATCGACAATGTGATGGGAATGATCGG20.1021972406745018No Hit
CACTATGAGAGCAAAATCGTCGGATGATTGGAGCCCATCCCACCAGTATA20.1021972406745018No Hit
CTTCAGGAATGTGAAGATTCCGTATATTGTATAAGTTTGGTCCTCCATCT20.1021972406745018No Hit
GATATATGTAATCATCGCCAGGAACATTCGAGGATTTTGATTTTCATTCC20.1021972406745018No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA20.1021972406745018No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG20.1021972406745018No Hit
GATACACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGA20.1021972406745018No Hit
ACATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTA20.1021972406745018No Hit
GTTTCAAGGCATGAATTCTCAAATATTCCTGGGTGGGATTCTTCAAGGAA20.1021972406745018No Hit
GTCCTTACGAATCCACTGTACCGGCTTCTGGTTGCCTTAGGGACAAGAGA20.1021972406745018No Hit
CCCCTGAATCCCTTTGTCAGTCATAAAGAGATTGATTCTGTAAACAATGC20.1021972406745018No Hit
GAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCATCATA20.1021972406745018No Hit
ACACTGAAGTTCGCTTTCAGTACTATGTTCTTTTCCATGATCGCCTGGTC20.1021972406745018No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCA20.1021972406745018No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA20.1021972406745018No Hit
AGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTC20.1021972406745018No Hit
CAAACAGACTGTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCC20.1021972406745018No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT20.1021972406745018No Hit
GTCAAAGAGAAAGACATGACCAAGGAATTCTTTGAAAACAAATCGGAAAC20.1021972406745018No Hit
CCCATTCGCTTCTGGTAGGCCTGCAAATTTTCAAGAAGGTCATCTTTCAG20.1021972406745018No Hit
CCCTGAGTCAGTGCTAGTCAACACTTATCAATGGATAATCAGGAACTGGG20.1021972406745018No Hit
ATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCAT20.1021972406745018No Hit
CTTCACATTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTA20.1021972406745018No Hit
GATTATCGGGGAAGACTTTGTAATCCCCTGAATCCCTTTGTCAGTCATAA20.1021972406745018No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG20.1021972406745018No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC20.1021972406745018No Hit
GGTTGGGAGAAGAGCAACAGCTATTCTCAGAAAGGCAACCAGGAGATTGA20.1021972406745018No Hit
TATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTTATTCAGTCTTTGT20.1021972406745018No Hit
GTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGAACAGTCTGGGCT20.1021972406745018No Hit
AACAAGTACAGTTCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGG20.1021972406745018No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT20.1021972406745018No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC20.1021972406745018No Hit
GACTAGGGAAAGGGTACATGTTCGAGAGTAAAAGAATGAAGATTCGAACA20.1021972406745018No Hit
GAATCCCACCCAGGAATATTTGAGAATTCATGCCTTGAAACAATGGAAGT20.1021972406745018No Hit
CCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTT20.1021972406745018No Hit
ATCATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTA20.1021972406745018No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG20.1021972406745018No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT20.1021972406745018No Hit
GCTTTTTTTATCGCTATGGATTTGTGGCTAATTTTAGCATGGAGCTACCC20.1021972406745018No Hit
GTGGAATAGGAATGGCCCAACAACAAGTACAGTTCATTACCCTAAGGTAT20.1021972406745018No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT20.1021972406745018No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)