FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_E12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_E12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATATAAATAATACATGGACATTTTAAGTCCAAAGCTTTTTTGATCGAT12.7777777777777777No Hit
CATGATCCGCTTGTCCTTTTGAAAGTGAACCCACCAAAACTGAAAGATGA12.7777777777777777No Hit
GTCTATTACTATCATTTTTCTATGAAACTGGGTTGTAATCTATATTTCCT12.7777777777777777No Hit
CATCAGTATCATTTCTCAAATGAGACCTTCCTTTTATAATGAACCCATAC12.7777777777777777No Hit
ATGTAGAACCAAAGAAGGAAGACGGAAAACAAACCTGTATGGGTTCATTA12.7777777777777777No Hit
CCTATATTAACTGATAGTTAAAGATTGGACTAATCTATTGAATACATTCG12.7777777777777777No Hit
NNGTATCGGGGAAGCCGTTGGCAGCCCCGGACTCCCGTGGGCAGTCGGGA12.7777777777777777No Hit
CTGATCAATGGATAATCAGGAACGGGGAAATTGTGCAAATTCCATGGGCC12.7777777777777777No Hit
ACTCGAGCCCATGCAAAGCTCGGTAGGGCCATGCTAAAAATAGCCACCAA12.7777777777777777No Hit
GCATCATATTCCATGCTTTTGGCTGGACCATGGGCTGGCATTACCACAGC12.7777777777777777No Hit
GTGTTGAACCCAGTGATTTTTAAAGAAATCTCTGTTTCTTGAGAATTAGT12.7777777777777777No Hit
GATGATCGGCTTGTCCTTTTGAAAGTGAACCCACCAAAACTGAAAGATGA12.7777777777777777No Hit
TCGTATACTTTGTTGAAACTTTAGCTAGGAGCATATGCGAAAAGCTTGAA12.7777777777777777No Hit
CATTAGACTGATCTCATGTCGAACTGTTTAGAAGGTAATTGAGTATTATT12.7777777777777777No Hit
CTGTGGAATTGCCTCTTACCAGTATCCTCAACCCTTCTCCTCTCACATTC12.7777777777777777No Hit
CATGATTGAGGCCGAGTCTTCTGTCAAAGAGAAAGACATGACCAAGGAAT12.7777777777777777No Hit
CTATAATACCTCTCATCTATATCTCCCTTAGTCCATACAAGTAGGCCAGT12.7777777777777777No Hit
CTCTTGAGGAGTGCGATAGGCCAAGAGTCGAGGACCATGTTCCTATAAGT12.7777777777777777No Hit
CCATCACATCCTTTAAGAAATCTATTAGCCTTCCTGACTCATTAGCTGTT12.7777777777777777No Hit
CCTATAAGCAATGCTAGGGAAAAATTTCTCGAATAGATTGAAGCACTTCT12.7777777777777777No Hit
GATCCGCAGTCAAGATAGAAGAAGAAGTTCTAACGGGCAACCTCCCAACC12.7777777777777777No Hit
GAGAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGG12.7777777777777777No Hit
ATCCTGTGCAGCCATGGATGACTTTCAGCTGATCCCAATGATAAGCAAAT12.7777777777777777No Hit
TTCTGTCATAATCAACCCTTGTACTTCGGCCAGTTTCACCCCTCCAGAAA12.7777777777777777No Hit
ATGTGATGGGAATGATCGGAATCCTGCCCGCCATGACCCCAAGCACGGAG12.7777777777777777No Hit
GTGTCATCATTTGAAAGGTTTGAGATATTCCCCAAGACAAGTTCATGGCC12.7777777777777777No Hit
GTATAAAAAAGGTAAATTTAATACAATTCTAAACTAATTGATGGCCATCC12.7777777777777777No Hit
GGTGGGGCAGCAGCAAAGGGGAGAATTTTTATTATGTGGACAGTGTAAAA12.7777777777777777No Hit
AGTGGCAGCAGCCCCATTAGCAGCTCTCTGGGAAATGTGCCAAAGCACCC12.7777777777777777No Hit
CTAAGGCATGGGTCAACACTGCCGAGAAGGGCTGGTGCCGCCGGGGGGTC12.7777777777777777No Hit
CTTGATGGTGGCGTACATGCTAGAAAGAGAATTGGTCCGTAAAACAAGGT12.7777777777777777No Hit
CAGATCCACCTATAGGTGCGATAGGGGAGCCACCCGACTTCAGACGAGAA12.7777777777777777No Hit
ATCTTTATCACAACATTTGCCAGTTTGGCCTTCTTATCATTGACCCCTAC12.7777777777777777No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC12.7777777777777777No Hit
AAATTAGACAATATAATTAATATTCAAATTCCGTAATGCTTCTTAAAATT12.7777777777777777No Hit
CCATGGATGAATGTCCGCTTAGCCCCATGATAAGCAAAGGTATGACCAAC12.7777777777777777No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)