FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_E07_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_E07_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3895
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG190.4878048780487805No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC130.3337612323491656No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA120.3080872913992298No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT110.28241335044929394No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT100.25673940949935814No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT90.23106546854942236No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC90.23106546854942236No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG90.23106546854942236No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA90.23106546854942236No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG80.20539152759948653No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG80.20539152759948653No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC80.20539152759948653No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA80.20539152759948653No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG70.1797175866495507No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG70.1797175866495507No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT70.1797175866495507No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC70.1797175866495507No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC60.1540436456996149No Hit
CCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGG60.1540436456996149No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA60.1540436456996149No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC60.1540436456996149No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC60.1540436456996149No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG50.12836970474967907No Hit
GGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGA50.12836970474967907No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC50.12836970474967907No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG50.12836970474967907No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT50.12836970474967907No Hit
CCATGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGA50.12836970474967907No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT50.12836970474967907No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA50.12836970474967907No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA40.10269576379974327No Hit
GTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATTTCAGA40.10269576379974327No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA40.10269576379974327No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA40.10269576379974327No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG40.10269576379974327No Hit
TACAAACCCTGGCCATGCAGATCTCAGTGCCAAGGAGGCACAGGATGTGA40.10269576379974327No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT40.10269576379974327No Hit
GCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAG40.10269576379974327No Hit
ATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAA40.10269576379974327No Hit
CCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATATCT40.10269576379974327No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT40.10269576379974327No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG40.10269576379974327No Hit
TCCTATATGTGAGAACCAATGGAACCTCCAAGATCAAGATGAAATGGGGC40.10269576379974327No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC40.10269576379974327No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG40.10269576379974327No Hit
CTACAGGACCTGCAAGTTAGTGGGAATCAACATGAGCAAAAAGAAGTCCT40.10269576379974327No Hit
GTCCACATATATTACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGAC40.10269576379974327No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC40.10269576379974327No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG40.10269576379974327No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA40.10269576379974327No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG40.10269576379974327No Hit
CATATAGGAACATGGGCCTCGACACTTGGCCTATCGCAGTCCTCAAGAGC40.10269576379974327No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT40.10269576379974327No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCATGC100.00682074145.07
CCATGCT100.00682074145.08
CCACCAT100.00682074145.05
CCTCCAC100.00682074145.02
TCCACCA100.00682074145.04
AATGCAG100.00682074145.05
Produced by FastQC (version 0.11.5)