FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_E06_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_E06_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2959
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA80.27036160865157144No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG50.16897600540723218No Hit
GTATTTATGTACACTCCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGA40.13518080432578572No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT40.13518080432578572No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG40.13518080432578572No Hit
CTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTTTGATTATC40.13518080432578572No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC40.13518080432578572No Hit
ATCTGAGGGGGCGTGGTGTCGTCCTCAAGAATGGTTCAATTTTGGCGTTC30.1013856032443393No Hit
GTATGGGTTCATTATAAAAGGAAGGTCTCATTTGAGAAATGATACTGATG30.1013856032443393No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA30.1013856032443393No Hit
GTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTAC30.1013856032443393No Hit
ATATGTAATCATCGCCAGGAACATTCGAGGATTTTGATTTTCATTCCACT30.1013856032443393No Hit
ATCTAATAAGAGCACTGACATTAAATACGATGACCAAAGATGCAGAGAGA30.1013856032443393No Hit
CTCCTGGAGTGTACATCTGCTCCCAGCACGTCCCTTGGGTTAAGTGCAAC30.1013856032443393No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA30.1013856032443393No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC30.1013856032443393No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC30.1013856032443393No Hit
CCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATG30.1013856032443393No Hit
GTAATCCCCTGAATCCCTTTGTCAGTCATAAAGAGATTGATTCTGTAAAC30.1013856032443393No Hit
ATATTACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGACACACATTC30.1013856032443393No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA30.1013856032443393No Hit
GCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAG30.1013856032443393No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG30.1013856032443393No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT30.1013856032443393No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC30.1013856032443393No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC30.1013856032443393No Hit
CCAATACTCATGTCAGCTGATTCATTTACTCCAGACACTCCAAAGCTGGG30.1013856032443393No Hit
CTGGTGGGGCAGCAGCAAAGGGGAGAAGTTTTATTATTTGGACAGTGTCA30.1013856032443393No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA30.1013856032443393No Hit
GCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTG30.1013856032443393No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACA100.0067389184145.04
GCATAAA100.0067389184145.01
Produced by FastQC (version 0.11.5)