FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_E05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_E05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG11.8867924528301887No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT11.8867924528301887No Hit
GAAAAACATCGTCGCGATTGCGGAAAATGCAATGAAAGAATATGGGGCAG11.8867924528301887No Hit
GTTGAATAGATCGCCAAAATGTGGTAATTCCTTCTTGCTTCCAGCTGTAC11.8867924528301887No Hit
CAAAGTCATTTGGAATCAAATTAGTAATATAAAAATCGAAAATAGTAACA11.8867924528301887No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCTCTGTCTTTCTGTCAGTAA11.8867924528301887No Hit
GTATGCTAGAGTACCGGTTTTAGATCATTAAACACAATAATGGACATTTG11.8867924528301887No Hit
CTCTTGCATCAATTATCTCAAATCGGAGCATCAAAAATGCATACGGGTAA11.8867924528301887No Hit
CAGTATGACAGTGATGAGCCAGAGCCCAGATCTCTAGCAAGCTGGGTCCA11.8867924528301887No Hit
GAGACTGAAATCGTTCAAATTCCATTTTGTTGTATAACATTGTGGGATCT11.8867924528301887No Hit
CATGATGAGTTAACTGATCATTCGGTTGAGTAACACCTAGGGTTGAATTC11.8867924528301887No Hit
GCCAGGAACATTCGAGGATTTTGATTTTCATTCCACTTAGTGTTGTCCCC11.8867924528301887No Hit
ATCTAATGTCGCAGTCCCGCACTCGAGAGATACTCACTAAGACCACTGTG11.8867924528301887No Hit
GTGAATAATGGACCTGACAGGTCTTATGTCGCAGTCCCGCAGTCGCGAGA11.8867924528301887No Hit
ATCTGAGAAGACACACATTCACATCTTTTCAGTCACTGGAGAGGAGATGG11.8867924528301887No Hit
ATCAAAAGGACAAGCTTATAATAAGTCAAGAAAGAAGAAGAAGTCCTAAC11.8867924528301887No Hit
CATATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT11.8867924528301887No Hit
AATGGAGGGAACGAATACTGATGGGGTTCTAGGTCTGTAGGTAAGTCAGT11.8867924528301887No Hit
CATATAGAAGACCCGTTGCAATTAAAACCAAGGTCCAGTCCATGCTTTCA11.8867924528301887No Hit
TTTGGACAGTGTCAAATGTACCAAGCACATCCCGCATTTGCTGGAACAGT11.8867924528301887No Hit
CGCCAAAATTGAACCATTCTTGAGGACGACACCACGCCCCCTCAGATTGC11.8867924528301887No Hit
AATTTAAAAAATTATACAATAATATATTGAAATAATAAATAGTTTTGACA11.8867924528301887No Hit
GTTTGTATCAACTCTCCTCCTTATTTTAACATGATTTCTGAAGTGGCCAG11.8867924528301887No Hit
GACACATACTGAGCTCATGCCTCAAAGGGTATCCTTGGAGACTCCACCGC11.8867924528301887No Hit
CTACGTACCCTTGCCCAATAACAACATTAGACTTCTCTCCTTTTGCAAGA11.8867924528301887No Hit
CACCAGCTCGGTTTAGGGGAGAAATCACAAACACAGCACGCAGACTCACA11.8867924528301887No Hit
CTTGAACGGAACGGACATCCTCTGGTGCAGCTCCTCCACGCGACACTCCA11.8867924528301887No Hit
GTCCTGCACACTTTCCAAATAGAGCCTTCCTCCACTCCCCTGGGTGACTC11.8867924528301887No Hit
ACTGCAAAGGCCCAGATGTATCTTAAATTGTTCATCAAAGACTACAGATA11.8867924528301887No Hit
CACTAAGACCGCGGTGGAGCTTTGGGCCCTAATCATAAAGTCGGCAGCAG11.8867924528301887No Hit
CTCCTGGGATGATGATGGGCATGTTCAACATCCTAAGTACGGTCTTGGGA11.8867924528301887No Hit
NNTTGATGGCCCTCCGCATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGG11.8867924528301887No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG11.8867924528301887No Hit
GAATAAAATAACTGATAGATCTAATGTAGAAGTACCGCACTAGCTATATA11.8867924528301887No Hit
CCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAA11.8867924528301887No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT11.8867924528301887No Hit
CATTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGG11.8867924528301887No Hit
GTAAAATCCATTATTATTAAGAAGGTCGAGACTATGTTACAGATACACAG11.8867924528301887No Hit
AGACAACACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTTCTAATT11.8867924528301887No Hit
TTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTG11.8867924528301887No Hit
CCCTCTTTGAGTGCGAATGGACCACGCGATCATCCATAAGAACATAGTAC11.8867924528301887No Hit
TCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGC11.8867924528301887No Hit
GGGGTCAGGTTATGCAGCCGACCAGTAGACCACACAGATTGCCAATGAGG11.8867924528301887No Hit
ATTCTTTATTGATCCGTACAGACTCGAACTCGACACTGTGATAAAACAGG11.8867924528301887No Hit
CCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGATAGCGAAA11.8867924528301887No Hit
GTCGCATTCACGAACAAGCCAGAAACTCACTAATACCACAATGGAACATA11.8867924528301887No Hit
CTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTG11.8867924528301887No Hit
TATCAATATTGAGTAGCAACTCAATTCAACTTGGTAATCAAAATCATATT11.8867924528301887No Hit
ACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGTTCCTG11.8867924528301887No Hit
CAGGTGATACAAACAAATGCACAGCATAGCTCAAATCCACCGCGGCACTC11.8867924528301887No Hit
GCCCGGCCCGCTGCGCCCGCTGGACGACCAAAGTCGCCCCCCGCCCGTAC11.8867924528301887No Hit
CTTCTTTCTTGATCCGTCCAGACTAGAAGTCGACCCTGGCATCAATCCGG11.8867924528301887No Hit
CACCAAAACTGAAAGATGAGCTAATCCTCAACCCTATTGCTGCCTTGCAT11.8867924528301887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGTCT50.0145.08
CCTGAAG50.0145.05
CATTCCT50.0145.01
TTCCTGA50.0145.03
CTGAAGT50.0145.06
TGAAGTC50.0145.07
ATTCCTG50.0145.02
TCCTGAA50.0145.04
AAGTCTG50.0145.09
AAGCATG50.0145.0145
Produced by FastQC (version 0.11.5)