FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_E05-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_E05-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1694092
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA6100.048.7850271
CGAAAGC7200.043.280093
GCGAAAG7350.040.4559442
GCGTACT1606.865978E-527.1670464
TCGCGTA1450.001224362925.0085092
GTATAAG7150.023.348251
GTATATA24000.022.9844911
TATAAGG6800.020.2642482
CGCGTAC1800.004318966520.1302743
GTATAAA13000.020.0997981
TAGACCG2256.91075E-419.3165055
TTATCGT1900.00591519819.0645944
GTATAGG6101.8189894E-1219.038061
TTATAGT10550.018.5405434
TATAGTG8600.018.530375
CTCGCGC2400.001058753318.12849145
GTATACG2851.6196806E-417.8273051
CTTATAG7350.017.7475073
TATACAG14600.017.3650115
GTATTAA8850.017.222991
Produced by FastQC (version 0.11.5)