FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_E03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_E03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAAGATACCAATGAATCAGAGATATGAAGAATTAAAAAATGTGGCGAG12.0408163265306123No Hit
CTCTGGTTGGGATACAGATCGGGGATTGGGTCTGGAAAGAGGAGTCCAGC12.0408163265306123No Hit
TCTCTTGACTGCGGATCCGCATCGGCTGTTAGCTGAGAATCCGCCACAAC12.0408163265306123No Hit
AATCTCTTTCTAGCATGTACGCCACCATCAAGGCAGCAATTTTACAATCC12.0408163265306123No Hit
ACCTTGTCGATGGCTTCCATTCCCCTGTTCTGGAAAAGCACTTGTGCATG12.0408163265306123No Hit
TTGCTTCTCTGAGTACAGTTTTGGAGATTACTAGCAACATAAGGTGAATG12.0408163265306123No Hit
ATGCTAATCCAGCCACCCGACGCAACATGCAAACACAGTACCTTCTAATT12.0408163265306123No Hit
CTGGTGATATATGTAATCATCGCCAGGAACATTCGAGGATTTTGATTTTC12.0408163265306123No Hit
GAAAATGAAGGTATGTATGGATTTACTTCCGGACTGTTAATTCTTTGCTG12.0408163265306123No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC12.0408163265306123No Hit
AGCTACATGTTCACCCAATCTCTTCCCTCTACCTGGCTAAATTGCTAGAT12.0408163265306123No Hit
GTTCTGGTGGGGCAGCAGCAAAGGGGAGAAGTTTTATTATTTGGACAGTG12.0408163265306123No Hit
AAATCCAGCTCCGACCTGTTCTCTTAATAACATTTATTGGAACTCACCCA12.0408163265306123No Hit
CTGCTTAACAGAACTATCTCCTGCCCTTTGTTCTTCCTGCTACAGCCTTA12.0408163265306123No Hit
ATGCTGTGCCATCTATTAGAAGAGGCCTTAGTTTGTCAATTTTCTTGTGT12.0408163265306123No Hit
GATGCAGAGAGAGGCAAGTTAAAAAGAAGGGCTATCGAAACACCTGGGAT12.0408163265306123No Hit
ATTAAATAACTATACATAAAAGAGCATAAAAATGAATATAACTGAAGCAA12.0408163265306123No Hit
CTATTGACGAAATTCAGATCACCTCTGACTGCTTTTATCATACAATCCTC12.0408163265306123No Hit
GAACACAGGTGAAGAACACGGCTCTTACTATTCAATGGATGATTGCCATG12.0408163265306123No Hit
GCTATTGTCATTCTCATCTCAGTAGACAGACAGTTCTGCCTTCGCTCCTA12.0408163265306123No Hit
CTAGATATGTGGTCAGTGGGAGTTATCGTCTATGTTAGCCTCAGTGGCAC12.0408163265306123No Hit
CAGTATGCTAGATGACCTCCCACAGATATACAAAGCTCTGTTCTTTTTTC12.0408163265306123No Hit
CTAGATATGTGGTCAGTGGGAGTTATCGTCTATGTTAGCCTCAGTGGCAG12.0408163265306123No Hit
CCACTATATGAGGCACTCAAATGCCTGAAGACATGCTTTGGCGGGAACGA12.0408163265306123No Hit
GTCTTACTCATATCATGATTTGGCATTCCAACCTCAATGATGACCCATAT12.0408163265306123No Hit
ATTCCAAAATGATCACTCAAGGAAGTAAGAATCTCAATAGTAGGATTCAC12.0408163265306123No Hit
AAAAAGGAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12.0408163265306123No Hit
ATAAATACCTTTGCAAAGGAGATACTTTCTTCTTTCTCAAAACGTTGTCC12.0408163265306123No Hit
GGGAGCAGAGGAGGACTTCTCACTTGACCTTCGGATTGCACTGCGGGGCA12.0408163265306123No Hit
GTCTATTAGACTACAGATAATCAGCCAAAATGATCTTCCTCACGAGACAC12.0408163265306123No Hit
CACTAACACTCTCTCCGTGCTGGAGTATTAATCTACTCCCATTTTGCTGA12.0408163265306123No Hit
GAATCTAACCAGAATGGGGTAGGCGGGGCCATGAGCCTTTTTGCTTTTTA12.0408163265306123No Hit
GGGCCATTGATCTCCCACATCATTGATGACGAATAAGTTATTGTCAACTT12.0408163265306123No Hit
NTAGATATGTGGTCAGTGGGAGTTATCGTCTATGTTAGCCTCAGTGGCAC12.0408163265306123No Hit
AGACAAGAAATGGCCAGTAGGAGTCTATGGGATTCCTTTCGTCAGTCCGA12.0408163265306123No Hit
GTTTTATATACCTTAGGTTAATGAACTGTACTTGATGTTTGGACATTACT12.0408163265306123No Hit
CGACTGTACCGTCTTCTGGTTGCCTTAGTGACAAGAGACTGAAATCGTTC12.0408163265306123No Hit
CTCATAAAGAGTATAAAAGACAATTCAACAAAAATTTTACATAACAGGAA12.0408163265306123No Hit
GCAGAAAAGAGAATAATGTAAATTATAAAAGAGAGTCATGAAAAAAGACA12.0408163265306123No Hit
GGGAGCTAAACTAACTCCAGGTCGCCAGACTTATGATTGGACATTACACA12.0408163265306123No Hit
TTGCTACATAGCTCAGAAGGCAAAGGCATCTAAATCACTACACCAAATTA12.0408163265306123No Hit
GATCCCCAACAATAGGCCCAAGAATTGTTAAATCAGTATTGGAAATCTAA12.0408163265306123No Hit
ACTTGATACAGTAATTTTAAAAAAAAGCAAGACCTATGCCTGCAAGGCCA12.0408163265306123No Hit
TTTATGTACAATCCCGTCATTATGTAGTCAGTCGCCATGCAGTGTGACAC12.0408163265306123No Hit
GCCTAACTTATTCAGTATTTGTTTTTTCTTCCCTAATGTTCATTGCCTGA12.0408163265306123No Hit
GTATAGCATTGTAGCTTTCTCTTACAACAAAATTTAAAAAGTTTCCCAGT12.0408163265306123No Hit
CAGATCTCTAGCAAGCTGGGTCCAAAATGAATTCAATAAGGCATGTGAAT12.0408163265306123No Hit
CTTGGCGCAGGGTTCCCGGCCAGAGTCTCACCGCGCCCTTGCCCTGACAG12.0408163265306123No Hit
CTAGATATGTGGGCAGTGGGAGTTATCGTCTATGTTAGCCTCAGTGGCAC12.0408163265306123No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)