FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_E02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_E02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1377
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA90.6535947712418301No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC90.6535947712418301No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG80.5809731299927379No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA60.4357298474945534No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT60.4357298474945534No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG50.36310820624546114No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG50.36310820624546114No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT40.29048656499636893No Hit
ATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG40.29048656499636893No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG40.29048656499636893No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG40.29048656499636893No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG40.29048656499636893No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA40.29048656499636893No Hit
CTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATATG40.29048656499636893No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG30.2178649237472767No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG30.2178649237472767No Hit
GAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCC30.2178649237472767No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC30.2178649237472767No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA30.2178649237472767No Hit
ATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGG30.2178649237472767No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG30.2178649237472767No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.2178649237472767No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT30.2178649237472767No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT30.2178649237472767No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT30.2178649237472767No Hit
CACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGT30.2178649237472767No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT30.2178649237472767No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG30.2178649237472767No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG30.2178649237472767No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC30.2178649237472767No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC30.2178649237472767No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGT20.14524328249818447No Hit
AAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAG20.14524328249818447No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC20.14524328249818447No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG20.14524328249818447No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA20.14524328249818447No Hit
TAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAA20.14524328249818447No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGA20.14524328249818447No Hit
ATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTT20.14524328249818447No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA20.14524328249818447No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACCCCATG20.14524328249818447No Hit
TTTCATTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCT20.14524328249818447No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT20.14524328249818447No Hit
CTCAGAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAA20.14524328249818447No Hit
CAGGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAG20.14524328249818447No Hit
AAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG20.14524328249818447No Hit
GGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGA20.14524328249818447No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG20.14524328249818447No Hit
GTAGCAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC20.14524328249818447No Hit
GAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGT20.14524328249818447No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGCCCCTGGCATCAATCCGG20.14524328249818447No Hit
GGGTGAATCAATAATTGTAGAATCTGGTGACCCGAATGCACTATTGAAGC20.14524328249818447No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA20.14524328249818447No Hit
GTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACGGGGTGAATC20.14524328249818447No Hit
ATGTTCCTATATGTGAGAACCAATGGAACCTCCAAGATCAAGATGAAATG20.14524328249818447No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG20.14524328249818447No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT20.14524328249818447No Hit
CCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTG20.14524328249818447No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA20.14524328249818447No Hit
GTGGTGAACAGTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCT20.14524328249818447No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA20.14524328249818447No Hit
ATATGCACACATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCAT20.14524328249818447No Hit
AGGAAGGAATGAACAAGGACAAACCCTCTGGAGCAAAACAAACGATACTG20.14524328249818447No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC20.14524328249818447No Hit
GCAATGAGATGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAA20.14524328249818447No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT20.14524328249818447No Hit
CTAACAAGTTTGCTGCAATATGCACACATTTGGAAGTTTGTTTCATGTAT20.14524328249818447No Hit
AACAAGTTTGCTGCAATATGCACACATTTGGAAGTTTGTTTCATGTATTC20.14524328249818447No Hit
ACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTG20.14524328249818447No Hit
TGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTC20.14524328249818447No Hit
ATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACT20.14524328249818447No Hit
GAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCA20.14524328249818447No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA20.14524328249818447No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA20.14524328249818447No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC20.14524328249818447No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT20.14524328249818447No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT20.14524328249818447No Hit
TCTTTGGTCATCGTATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTT20.14524328249818447No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG20.14524328249818447No Hit
CAGTAAACAGAACACACCAATACTCAGAAAATTCGAGAAATTTTTCCCTA20.14524328249818447No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC20.14524328249818447No Hit
TCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGC20.14524328249818447No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG20.14524328249818447No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)