FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S2_R2_E01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S2_R2_E01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCGGAATGCTCTAATAGAACTATTAGTTCTTGATCCGTCCAGCATCGC13.8461538461538463No Hit
GAGCAAAAGCTATTCTCAGAAAGTAAACCAGTAGATTGATCCAGTTGATA13.8461538461538463No Hit
CTTCTTTCTTCTCTTTTGTTATTGCCAGCTGTGACTCTGATGTCAGTATT13.8461538461538463No Hit
GTATGGATAGCAAATAGTAGAATTGCCACAACTACTTCACTGAATGAGTG13.8461538461538463No Hit
AGCATGACACATGATGCAGGGAGCGGCAAGTGAATTAGCGGGGCTATCGA13.8461538461538463No Hit
CCGAGGACGCCGCAATGATATGACGGACAACCGGGCAATGGTTGTGCGCA13.8461538461538463No Hit
GAATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAATGGACATG13.8461538461538463No Hit
ACAAAGTTCAGATCGCCCCTAACTGCCTTGATCATGCAATCCTCCTGTGA13.8461538461538463No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT13.8461538461538463No Hit
CCATAATGTTCTCAAATAAAATGGCAAGACTAGGGAAAGGGTACATGTTC13.8461538461538463No Hit
TGTTGATACCCACTAACTTGCAGGTCCTGTAGAATCTGTCCACTCCTGCT13.8461538461538463No Hit
ATCAGAATGATCAAACGTGGAATCAATGACCGATATTTCTGGAGGGTTGA13.8461538461538463No Hit
GGAGCATTTGCGAAAAGCTTGAACAGTCTGGGCTCCCAGTAGGGGGCAAT13.8461538461538463No Hit
AAATAGTTCCTCCTCATGCTTGCGATATGTTCAATCGGGGCAACATCTTC13.8461538461538463No Hit
GAATAAGAGCGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA13.8461538461538463No Hit
GAAGAGCTCCAGGATTGTAAAATTGCTCCCTTGATGGTGGCGTACATGCT13.8461538461538463No Hit
GCCCCCTACTGGGAGCCCAGACTGATCAAGCTTTTAGCAAATGCTCCTAG13.8461538461538463No Hit
CACTATGAGAGCAAAATCGTCGGATGATTGGAGCCCATCCCACCAGTATA13.8461538461538463No Hit
GCTTGGGGTCATGTCGGGCAGTATTCCGATCATTCCCATCACATTGTCGA13.8461538461538463No Hit
TTCCGTATATTGTATAAGTTTGGTCCTCGATCTGATACTAATAGCCCTAC13.8461538461538463No Hit
GTGTTGAACCTTGCATTAGAGAGCACATTCTGGGATCCATTCCGGTGCGA13.8461538461538463No Hit
CTGCAAAGATGTTGGAGACCTTAAACAGTATGACAGTGATGAGCCAGAGC13.8461538461538463No Hit
TATTATCCCTCTCAGCGACATATCCGTGCTTGGGGTCATGTCTGGCAGTA13.8461538461538463No Hit
GACATGGCCCCCCCCTAGCCTGAGCCATTCCACAGCCCTCGGGTCACTAT13.8461538461538463No Hit
GGAAATGAAGGTCCTGAGCCGTAGTGGAGGCCTTTTTTCTACCTGGATTC13.8461538461538463No Hit
CTGTGTCTTGTGAATTAGTCATCATCTTTCTCACAACATTTGCCAGTTTG13.8461538461538463No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)